# US 6,720,136 B2 · Solid phase quenching systems

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- Number: US 6,720,136 B2
- Document ID: US6720136B2
- Status: Granted
- Kind: B2
- Application number: 10/166,898
- Filed: 2002-06-11
- Published: 2002-10-17
- Granted: 2004-04-13
- Earliest claimed: 1998-09-25
- Assignees: V. I. Technologies, Inc.
- Inventors: Andrei A. Purmal; Samuel K. Ackerman
- Web page: https://patentgrep.com/patent/US6720136B2

## CPC

- C07F 9/1651: {with hydroxyalkyl compounds with further substituents on alkyl}
- A01N 1/12: Chemical aspects of preservation
- A01N 1/124: Disinfecting agents, e.g. antimicrobials
- A01N 1/16: Physical preservation processes
- A61K 35/14: Blood; Artificial blood (perfluorocarbons A61K31/02; umbilical cord blood A61K35/51; haemoglobin A61K38/42)
- A61K 35/16: Blood plasma; Blood serum (umbilical cord blood A61K35/51)
- A61M 1/3687: {Chemical treatment (A61M1/3675 takes precedence)}
- C07F: ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM (metal-containing porphyrins C07D487/22)
- A01N: PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF (preservation of food or foodstuff A23); BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES (preparations for medical, dental or toiletry purposes which kill or prevent the growth or proliferation of unwanted organisms A61K); PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)
- A61M: DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY (introducing media into or onto the bodies of animals A61D7/00; means for inserting tampons A61F13/26; devices for administering food or medicines orally A61J; containers for collecting, storing or administering blood or medical fluids A61J1/05); DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY (surgery A61B; chemical aspects of surgical articles A61L); DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR {(Electrotherapy, e.g. producing anaesthesia by the use of alternating or intermittent currents A61N1/36021)}

## Abstract

Methods of inactivating contaminants of a biological matrix are disclosed. The methods include the steps of: (a) contacting a biological matrix with an inactivating agent including an aziridino moiety, where a portion of the agent reacts with and inactivates the contaminant, and a portion of the agent remains unreacted; (b) contacting the product of step (a) with a solid support including at least 1 quenching moiety attached to the solid support through covalent bonds, under conditions and for a time sufficient to allow the unreacted agent to bond covalently to the quenching moiety; and (c) separating the solid support and the unreacted agent from the biological matrix, where the unreacted agent is attached to the solid support through covalent bonds.

## Claims

### Claim 1

A method of quenching an electrophile, said method comprising contacting said electrophile with a solid support comprising at least 1 thiophosphate moiety attached to said solid support through covalent bonds, under conditions and for a time sufficient to allow said electrophile to bond covalently to said thiophosphate moiety.

### Claim 2 (depends on 1)

The method of claim 1, wherein said solid support comprises at least 2 mmol/g thiophosphate moieties.

### Claim 3 (depends on 1)

The method of claim 1, wherein said solid support comprises at least 100 mmol/g thiophosphate moieties.

### Claim 4 (depends on 1)

The method of claim 1, wherein a plurality of said thiophosphate moieties are substituted with at least one C1-12 saturated or unsaturated hydrocarbon skeleton that is unsubstituted or has between 1 and 4, inclusive, substituents, independently selected from the group consisting of hydroxyl, amino, cyano, and azido.

### Claim 5 (depends on 1)

The method of claim 1, wherein said electrophile comprises an aziridino moiety.

### Claim 6 (depends on 1)

The method of claim 1, wherein said electrophile is ethyleneimine.

### Claim 7 (depends on 1)

The method of claim 1, wherein said electrophile is an oligomer of ethyleneimine.