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US 6,720,014 B1Granted
Phytase-containing foodstuffs and methods of making and using them
AssigneeDiversa Corporation
InventorsJay M. Short, Keith A. Kretz
Life of this patent
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Abstract
A purified recombinant phytase enzyme derived from Escherichia coli B. The enzyme has a molecular light of about 47.1 kilodaltons and has phytase activity. The enzyme can be produced from native or recombinant host cells and can be used to aid in the digestion of phytate where desired. In particular, the phytase of the present invention can be used in foodstuffs to improve the feeding value of phytate rich ingredients.
Claims40
A method to produce a foodstuff comprising a polypeptide having a phytase activity comprising:
- (a) providing a cell comprising an isolated or a recombinant phytase-encoding nucleic acid having a nucleotide sequence selected from the group consisting of
- (i) a sequence as set forth in SEQ ID NO:
- 1,
- (ii) a sequence as set forth in SEQ ID NO:
- 1, wherein T can also be U;
- and
- (iii) a nucleic acid encoding a polypeptide as set forth in SEQ ID NO:2 or a phytase having a sequence as set forth in SEQ ID NO:2 with conservative amino acid substitutions, wherein conservative amino acid substitutions comprise replacements, one for another, among the aliphatic amino acids Ala, Val, Leu and Ile, or, interchange of the hydroxyl residues Ser and Thr, or, exchange of the acidic residues Asp and Glu, or, substitution between the amide residues Asn and Gln, or, exchange of the basic residues Lys and Arg, or, replacements among the aromatic residues Phe, Tyr, or any combination thereof, or active fragments thereof;
- (b) culturing the cell under conditions wherein said nucleic acid is expressed as a polypeptide having a phytase activity;
- and
- (c) converting said cell into a composition suitable for a foodstuff.
7 more elements
A method to produce a foodstuff containing a phytase comprising:
- (a) providing a plant cell, plant part, seed or plant comprising a phytase-encoding nucleic acid having a nucleotide sequence selected from the group consisting of
- (i) a nucleic acid comprising a sequence encoding a polypeptide sequence as set forth in SEQ ID NO:2 and
- (ii) a nucleic acid comprising a sequence encoding the polypeptide sequence as set forth in SEQ ID NO:2, wherein T can also be U;
- (b) culturing the plant cell, plant part, seed or plant under conditions wherein said nucleic acid is expressed and a phytase is generated;
- and
- (c) converting said plant cell, plant part, seed, or plant into a composition suitable for foodstuff.
3 more elements
Dependencies are inferred from the claim text.