In situ crystal growth and crystallization
AssigneeSyrrx, Inc.
InventorsPeter R. David, Nathaniel E. David
Life of this patent
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Abstract
A method is provided for determining crystallization conditions for a material, the method comprising: taking a plurality of different crystallization samples in an enclosed microvolume, the plurality of crystallization samples comprising a material to be crystallized and crystallization conditions which vary among the plurality of crystallization samples; allowing crystals of the material to form in plurality of crystallization samples; and identifying which of the plurality of crystallization samples form crystals.
Claims38
A method for determining crystallization conditions for a protein, the method comprising:
- within a microfluidic device, delivering material to an enclosed microvolume via one or more lumens that each have a cross sectional diameter of less than 500 microns to form a plurality of different crystallization samples within the enclosed microvolume, the plurality of different crystallization samples comprising a protein to be crystallized and crystallization conditions which vary among the plurality of different crystallization samples;
- allowing crystals of the protein to form in the plurality of crystallization samples within the microfluidic device;
- and
- identifying which of the plurality of crystallization samples within the microfluidic device comprise a precipitate or a crystal of the protein.
A method for determining crystallization conditions for a protein, the method comprising:
- within a microfluidic device, delivering material to a plurality of enclosed microvolumes via one or more lumens that each have a cross sectional diameter of less than 500 microns to form a plurality of different crystallization samples within the plurality of enclosed microvolumes, each microvolume comprising two or more crystallization samples, the different crystallization samples comprising a protein to be crystallized and crystallization conditions which vary among the plurality of different crystallization samples;
- allowing crystals of the protein to form in the plurality of crystallization samples;
- and
- identifying which of the plurality of crystallization samples comprise a precipitate or a crystal of the protein.
Dependencies are inferred from the claim text.