Dipstick for carbohydrate-free transferrin assay
AssigneeAxis-Shield ASA
InventorErling Sundrehagen
Life of this patent
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Abstract
The invention relates to a new dipstick assay for detecting and quantifying the content of an target analyte in a sample. The assay is particularly useful for example in the diagnosis and monitoring of alcoholism by the detection of asialo transferrin or carbohydrate free transferrin (CFT). Thus, provided is a dipstick for determining the content of a target analyte variant in a mixture of analyte variants in a sample, comprising: a) a sample application zone, b) a screening zone having an immobilised binding ligand having a binding affinity for a non-target analyte variant or varinats, c) a conjugate zone comprising a detector reagent, d) a reading zone for detection of said analyte.
Claims14
A dipstick for determining the content of a target analyte variant in a mixture of analyte variants in a sample, comprising:
- (a) a zone for contacting a sample with a binding ligand having a binding affinity for a non-target analyte variant, to allow separation of a non-binding fraction containing the target analyte variant;
- (b) a zone for determining the analyte content of the non-binding fraction, wherein said target analyte variant is a carbohydrate-free variant of said analyte,
- and wherein said carbohydrate free variant is carbohydrate-free transferrin (CFT) or asialotransferrin.
A dipstick for determining the content of a target analyte variant in a mixture of analyte variants in a sample, comprising:
- (i) a sample application zone,
- (ii) A screening zone having an immobilized binding ligand having a binding affinity for a non-target analyte variant or variants,
- (iii) a conjugate zone comprising a detector reagent, wherein said detector reagent comprises a second ligand having a binding affinity for the analyte and said second ligand is an anti-transferrin antibody or a fragment thereof,
- (iv) a reading zone for detection of said analyte,
- wherein said target analyte variant is a carbohydrate-free variant of said analyte which is a carbohydrate-free transferrin (CFT) or asialotransferrin.
Dependencies are inferred from the claim text.