Electrochemical test strip for use in analyte determination
AssigneeLifeScan, Inc.
InventorsYeung Siu Yu, Mahesh Shah
Life of this patent
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Abstract
Electrochemical test strips and methods for their use in the detection of an analyte in a physiological sample are provided. The subject test strips have a reaction zone defined by opposing metal electrodes separated by a thin spacer layer. The metal surface of at least one of the electrodes is modified by a homogenous surface modification layer made up of linear self-assembling molecules having a first sulfhydryl end group and a second sulfonate end group separated by a short chain alkyl linking group, where 2-mercaptoethane sulfonic acid or a salt thereof is preferred in certain embodiments. The subject electrochemical test strips find application in the detection of a wide variety of analytes, and are particularly suited for use the detection of gluose.
Claims22
An electrochemical test strip comprising:
- (a) a reaction zone defined by opposing working and reference electrodes separated by a spacer layer, wherein at least one of said working and reference electrodes has a surface modified with a homogenous surface modification layer made up of self assembling molecules having a first sulfhydryl end group and a second sulfonate end group, wherein said sulfhydryl and sulfonate end groups are separated by a lower alkyl linker group wherein all the self-assembling molecules are identical;
- and
- (b) a redox reagent system present in said reaction zone, wherein said redox reagent system comprises at least one enzyme and a mediator.
An electrochemical test strip comprising:
- (a) a reaction zone defined by opposing working and reference electrodes comprising a metal surface separated by a thin spacer layer, wherein at least one of said working and reference electrodes has a surface modified with a homogenous surface modification layer made up of self assembling molecules wherein all the self-assembling molecules are identical of the formula:
- HS—(CH2)n—SO3Y
- wherein:
- n is an integer from 1 to 6;
- and
- Y is H or a cation;
- and
- (b) a redox reagent system present in said reaction zone, wherein said redox reagent system comprises enzymes and a mediator.
An electrochemical test strip for use in detecting the concentration of glucose in a physiological sample, said test strip comprising:
- (a) a reaction zone defined by opposing working and reference electrodes each comprising a metal surface selected from the group consisting of gold and palladium separated by a thin spacer layer, wherein at least one of said working and reference electrodes has a surface modified with a homogenous surface modification layer made up of 2-mercaptoethane sulfonic acid or a salt thereof wherein all the self-assembling molecules are identical;
- and
- (b) a redox reagent system present in said reaction zone, wherein said redox reagent system comprises a glucose oxidizing enzyme and a mediator.
A method of determining the concentration of an analyte in a physiological sample, said method comprising:
- (a) applying said physiological sample to an electrochemical test strip comprising:
- (i) a reaction zone defined by opposing working and reference metallic electrodes separated by a spacer layer, wherein at least one of said metallic working and reference electrodes has a surface modified with a homogenous surface modification layer made up of self assembling molecules having a first sulfhydryl end group and a second sulfonate and group, wherein said, sulfhydryl and sulfonate end groups are separated by a lower alkyl linker group wherein all the self-assembling molecules are identical;
- and
- (ii) a redox reagent system present in said reaction zone, wherein said redox reagent system comprises at least one enzyme and a mediator;
- (b) detecting an electrical signal in said reaction zone using said metallic electrodes;
- and
- (c) measuring said detected electrical signal to thereby determine the concentration of said analyte in said sample.
Dependencies are inferred from the claim text.