Method for the iontophoretic non-invasive determination of the in vivo concentration level of an inorganic or organic substance

A method of making an iontophoretic device for use in sampling of glucose, said method comprising:

• positioning a positive electrode and a negative electrode;
• positioning a first collection reservoir, comprising an electrically conductive material, to be capable of electrical communication with said positive electrode or said negative electrode;
• positioning electrical leads for the positive electrode and the negative electrode, said leads capable of creating an electrical circuit;
• providing means for correlating a signal from glucose with an amount or concentration of glucose;
• providing a display to indicate said amount or concentration of glucose;
• and
• assembling said electrodes, collection reservoir, leads, means for correlating, and display to result in making said iontophoretic device.

A method of making monitoring system for use in sampling of glucose, said method comprising:

• assembling an iontophoretic device comprising:
• (A) positioning electrical leads for a positive electrode and a negative electrode and connecting said electrical leads for the positive electrode and the negative electrode to a power supply, said leads capable of creating an electrical circuit;
• (B) providing means for correlating a signal from extracted glucose with an amount or concentration of glucose;
• (C) providing a display, to indicate said amount or concentration of glucose, and positioning said display in operative communication with said means for correlating;
• and
• (D) assembling said leads, means for correlating, and display to make said iontophoretic device;
• assembling a component for use in said iontophoretic device, comprising:
• (a) positioning the positive electrode, the negative electrode, and an electrically insulating material separating the electrodes to present a single common surface;
• (b) positioning said positive and negative electrodes to be capable of operative communication with said electrical leads;
• (c) positioning a first collection reservoir, comprising an electrically conductive material, to be capable of electrical communication with said positive electrode;
• (d) positioning a second collection reservoir, comprising an electrically conductive material, to be capable of electrical communication with said positive electrode;
• providing an analyzing component in operative communication with said means for correlating;
• and
• assembling said iontophoretic device, said component for use in said iontophoretic device, and said analyzing component to make said monitoring system.