Invertase inhibitors and methods of use

A transformed plant having incorporated into its genome a DNA molecule, said molecule comprising a nucleotide sequence operably linked to a promoter capable of driving expression of a gene in a plant cell, wherein said nucleotide sequence is selected from the group consisting of:

• a) a sequence encoding the amino acid sequence set forth in SEQ ID NO:2;
• b) the nucleotide sequence set forth in SEQ ID NO:1;
• c) a nucleotide sequence having at least 95% sequence identity to the sequence set forth in SEQ ID NO:1, wherein said nucleotide sequence encodes a polypeptide having invertase inhibitor activity;
• d) a nucleotide sequence that encodes an amino acid sequence having 95% sequence identity to the sequence of SEQ ID NO:2;
• and
• e) nucleotide sequence that is a complement of any one of a)-d).

A method for modulating invertase activity in a plant, said method comprising transforming said plant with a DNA construct, said construct comprising a promoter that drives expression in a plant cell operably linked with a nucleotide sequence selected from the group consisting of:

• a) a sequence encoding the amino acid sequence set forth in SEQ ID NO:2;
• b) the nucleotide sequence set forth in SEQ ID NO:1;
• c) a nucleotide sequence having at least 95% sequence identity to the sequence set forth in SEQ ID NO:1, wherein said nucleotide sequence encodes a polypeptide having invertase inhibitor activity;
• d) a nucleotide sequence that encodes an amino acid sequence having 95% sequence identity to the sequence of SEQ ID NO:2;
• and
• e) nucleotide sequence that is a complement of any one of a)-d).

A method for increasing seed yield in a plant, said method comprising transforming said plant with a DNA construct, said construct comprising a promoter that drives expression in a plant cell operably linked with a nucleotide sequence selected from the group consisting of:

• a) a sequence encoding the amino acid sequence set forth in SEQ ID NO:2;
• b) the nucleotide sequence set forth in SEQ ID NO:1;
• c) a nucleotide sequence having at least 95% sequence identity to the sequence set forth in SEQ ID NO:1, wherein said nucleotide sequence encodes a polypeptide having invertase inhibitor activity;
• d) a nucleotide sequence that encodes an amino acid sequence having 95% sequence identity to the sequence of SEQ ID NO:2;
• and
• e) nucleotide sequence that is a complement of any one of a)-d).

A transformed plant cell having incorporated into its genome a DNA molecule, said molecule comprising a promoter capable of driving expression of a gene in a plant cell operably linked to a nucleotide sequence selected from the group consisting of:

• a) a sequence encoding the amino acid sequence set forth in SEQ ID NO:2;
• b) the nucleotide sequence set forth in SEQ ID NO:1;
• c) a nucleotide sequence having at least 95% sequence identity to the sequence set forth in SEQ ID NO:1, wherein said nucleotide sequence encodes a polypeptide having invertase inhibitor activity;
• d) a nucleotide sequence that encodes an amino acid sequence having 95% identity to the sequence of SEQ ID NO:2;
• and
• e) nucleotide sequence that is a complement of any one of a)-d).