Chimeric oligonucleotides for modulating gene expression
AssigneesIsis Pharmaceuticals, Inc., Emory University
InventorsRussell M. Medford, Clarence Frank Bennett
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Abstract
Genes coding for vascular cell adhesion molecules, particularly VCAM-1, are modulated through interaction of oligonucleotides with transcriptional regulatory factors which bind to the genes. Specific and effective oligonucleotides are provided which interact with the transcriptional regulatory factors to diminish their interaction with the genes and downregulate their function. Multi-modal oligonucleotides are also provided which interact both with a transcriptional regulatory factor and with another aspect of gene function.
Claims9
A linear chimeric oligonucleotide for down-regulating expression of a gene comprising a first region having a sequence which is identical to at least a portion of a sequence of said gene, which gene sequence binds a transcriptional regulatory factor, and a second region specifically hybridizable either with a splicing region of pre-mRNA deriving from the gene or with mRNA deriving from the gene.
A linear chimeric oligonucleotide for down-regulating expression of a gene comprising a first region having a sequence which is identical to at least a portion of a sequence of a gene, which gene sequence binds a transcriptional regulatory factor, a second region specifically hybridizable with a splicing region of pre-mRNA deriving from the gene, and a third region specifically hybridizable with mRNA deriving from the gene.
Dependencies are inferred from the claim text.