Method for removing endotoxin from the samples containing basic protein
AssigneeSamyang Genex Corporation
InventorsSang-Hyun Pyo, Heung-Bok Park, Jin-Eon So, Jin-Hyun Kim, Seung-Suh Hong, Hyun-Soo Lee
Life of this patent
Estimating the term…
Abstract
The present invention relates to a method of removing an endotoxin from solution containing basic proteins. More specifically, the method of the present invention comprises the steps of adding the surfactant to solution containing the basic protein obtained from the recombinant microorganism and mixing the resultant, of loading the resultant solution on the cation exchange column, washing the cation exchange column with solution which does not contain surfactant, and eluting the basic protein of interest from the cation exchange column.
Claims17
A method of removing endotoxin from a basic protein comprising the steps of:
- (a) adding a non-ionic detergent to a first solution containing the basic protein obtained from a recombinant microorganism to yield a resultant solution, and mixing the resultant solution,
- (b) loading the resultant solution on a cation exchange resin;
- (c) washing the cation exchange resin with a second solution that does not contain the detergent;
- and
- (d) eluting the basic protein from the cation exchange resin.
A method of removing endotoxin from a basic protein comprising the steps of:
- (a) adding a non-ionic detergent to a first solution containing the basic protein obtained from a recombinant microorganism to yield the resultant solution, and mixing the resultant solution, wherein the resultant solution consists essentially of a buffer solution, the non-ionic detergent, and the basic protein with the endotoxin;
- (b) loading the resultant solution on a cation exchange resin;
- (c) washing the cation exchange resin with a second solution which does not contain the detergent;
- and
- (d) eluting the basic protein from the cation exchange resin.
Dependencies are inferred from the claim text.