Human antibodies derived from immunized xenomice
OwnerRaju Kucherlapati, Aya Jakobovits +3inventors
InventorsRaju Kucherlapati, Aya Jakobovits, Daniel G. Brenner, Daniel J. Capon, Sue Klapholz
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Abstract
Fully human antibodies against a specific antigen can be prepared by administering the antigen to a transgenic animal which has been modified to produce such antibodies in response to antigenic challenge, but whose endogenous loci have been disabled. Various subsequent manipulations can be performed to obtain either antibodies per se or analogs thereof.
Claims14
An isolated human IgG2 monoclonal antibody or an antigen binding portion thereof that specifically binds IL-8.
An isolated human monoclonal antibody or antigen binding portion thereof that specifically binds IL-8, wherein the heavy chain comprises an amino acid sequence derived from a human VH gene selected from the group consisting of:
- 4-21, 4-34, 3-30 and 5-51.
An isolated antibody or antigen-binding portion thereof that specifically binds IL-8, wherein the heavy chain comprises an amino acid sequence encoded by a nucleic acid sequence selected from the group consisting of SEQ ID NOS:
- 10, 12, 14, 16 and a degenerate nucleotide sequence of SEQ ID NOS:10, 12, 14, and 16, wherein said degenerate nucleotide sequence encodes the same amino acid sequence encoded by SEQ ID NOS:
- 10, 12, 14, or 16.
An isolated antibody or antigen-binding portion thereof that specifically binds IL-8, wherein the light chain comprises an amino acid sequence encoded by a nucleic acid sequence selected from the group consisting of SEQ ID NOS:
- 11, 13, 15, 17 and a degenerate nucleotide sequence of SEQ ID NOS:
- 11, 13, 15, 17, wherein said degenerate nucleotide sequence encodes the same amino acid sequence encoded by SEQ ID NOS:
- 11, 13, 15, or 17.
An isolated antibody that specifically binds IL-8, comprising:
- (a) a heavy chain comprising an amino acid sequence encoded by SEQ ID NOS:10, 12, 14 or 16, and
- (b) a kappa light chain comprising an amino acid sequence encoded by SEQ ID NOS:11, 13, 15 or 17.
An antibody or antigen binding portion thereof that specifically binds IL-8 selected from the group consisting of:
- (a) an antibody wherein the heavy chain comprises an amino acid sequence encoded by SEQ ID NO:10, and wherein the light chain comprises an amino acid sequence encoded by SEQ ID NO:11;
- (b) an antibody wherein the heavy chain comprises an amino acid sequence encoded by SEQ ID NO:12, and wherein the light chain comprises an amino acid sequence encoded by SEQ ID NO:13;
- (c) an antibody or antigen binding portion thereof wherein the heavy chain comprises an amino acid sequence encoded by SEQ ID NO:14, and wherein the light chain comprises an amino acid sequence encoded by SEQ ID NO:15;
- and
- (d) an antibody or antigen binding portion thereof wherein the heavy chain comprises an amino acid sequence encoded by SEQ ID NO:16, and wherein the light chain comprises an amino acid sequence encoded by SEQ ID NO:17.
An antibody or an antigen binding portion thereof that specifically binds IL-8, selected from the group consisting of D1.1, K2.2, K4.2 and K4.3.
Dependencies are inferred from the claim text.