Method for identification of flunitrazepam
AssigneeR. E. Davis Chemical Corporation
InventorArthur J. Friedman
Life of this patent
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Abstract
A method for detecting the presence of flunitrazepam in a sample. Flunitrazepam contained within the sample is reduced and hydrolyzed by the addition of a hydrolyzing agent and a reducing agent. A visualizing agent is provided to combine with the reduced and hydrolyzed species corresponding to the flunitrazepam to yield a colored species providing a qualitative colorimetric indication of the presence of flunitrazepam within the sample. The invention is a rugged and dependable method suitable for use in the field.
Claims31
A method for detecting the presence of flunitrazepam in a sample, comprising the steps of:
- obtaining a sample suspected to contain flunitrazepam;
- simultaneously reducing and hydrolyzing flunitrazepam contained within the sample to a reduced and hydrolyzed species by the addition of a hydrolyzing agent and a reducing agent to the sample;
- and
- adding a visualizing agent to the sample which chemically reacts with the reduced and hydrolyzed species of the flunitrazepam to provide a colorimetric indication of the presence of flunitrazepam within the sample.
A method for detecting the presence of flunitrazepam in a sample, comprising the steps of:
- obtaining a sample suspected to contain flunitrazepam;
- reducing the flunitrazepam contained within the sample to a reduced species by the addition of a reducing agent to the sample;
- hydrolyzing the flunitrazepam contained within the sample to a hydrolyzed species by addition of a hydrolyzing agent to the sample;
- and
- adding a visualizing agent to the sample which chemically reacts with the both reduced and hydrolyzed species of the flunitrazepam to provide a colorimetric indication of the presence of flunitrazepam within the sample.
A method for detecting the presence of flunitrazepam in a sample, comprising the steps of:
- obtaining a sample suspected to contain flunitrazepam;
- reducing and hydrolyzing flunitrazepam contained within the sample to a reduced and hydrolyzed species;
- and
- adding a visualizing agent to the sample which chemically reacts with the reduced and hydrolyzed species of the flunitrazepam to provide a colorimetric indication of the presence of flunitrazepam within the sample.
A method for detecting the presence of flunitrazepam in a sample, comprising the steps of:
- obtaining a sample suspected to contain flunitrazepam;
- simultaneously reducing and hydrolyzing flunitrazepam contained within the sample to a reduced and hydrolyzed species by the addition of a hydrolyzing agent and a reducing agent to the sample wherein the simultaneous reducing and hydrolyzing is carried out at ambient temperature, the hydrolyzing agent is hydrochloric acid, and the reducing agent is zinc, tin, magnesium, iron, cobalt, copper, bismuth, lead or indium;
- and
- subsequently adding a visualizing agent to the sample which chemically reacts with the reduced and hydrolyzed species of the flunitrazepam to provide a colorimetric indication of the presence of flunitrazepam within the sample, wherein the reducing agent and hydrolyzing agent are provided at concentrations to effectively reduce and hydrolyze flunitrazepam in the sample to the reduced and hydrolyzed species in an amount sufficient to provide a concentration of reduced and hydrolyzed species capable of being calorimetrically detected following Schiff base formation with the visualizing agent wherein the flunitrazepam is present in the sample at a concentration of about 10 ppm or greater.
Dependencies are inferred from the claim text.