Method for the mass propagation of adventitious roots of ginseng, camphor ginseng and wild ginseng by tissue culture and the improvement of their saponin content

A method for improving saponin content of adventitious root of ginseng, camphor ginseng or wild ginseng, when culturing ginseng, camphor ginseng or wild ginseng using tissue culture, the method comprises the steps of:

• pre-treating the adventitious root with any one of the growth regulators BA(benzyl adenine), 2iP, zeatin, methyl jasmonic acid, TDZ, kinetin, or jasmonic acid at a concentration of 1.0-100 mg/L for 1-10 hours;
• obtaining the adventitious root by tissue culturing one of the ginseng, camphor ginseng and wild ginseng;
• seeding the adventitious root, which was pre-treated with any one of the growth regulators, on MS medium containing 3% of sugar and 0.5-5.0 mg/L of IBA or NAA and incubating it in a bioreactor in which the temperature is 22-25° C. and pH is 6.0 under any one of lights selected from the group consisting of blue light, red light, and fluorescent light;
• and
• harvesting the cultured adventitious root that was treated 10 days before harvest with 1.0-10.0 mg/L of jasmonic acid or methyl jasmonic acid in a bioreactor for 7 days.

A method of culturing adventitious root of ginseng, camphor ginseng or wild ginseng by tissue, the method for improving the saponin content of adventitious root of ginseng, camphor ginseng or wild ginseng by tissue comprises the steps of:

• pre-treating the adventitious root with any one of the growth regulators BA, 2iP, zeatin, methyl jasmonic acid, TDZ, kinetin, or jasmonic acid at a concentration of 1.0-100 mg/L for 1-10 hours;
• obtaining the adventitious root by tissue culturing one of the ginseng, camphor ginseng and wild ginseng;
• seeding the adventitious root, which was pre-treated with any one of the growth regulators, on MS medium containing 3% of sugar and 0.5-5.0 mg/L of IBA or NAA and incubating it in a bioreactor which the temperature is 22-25° C. and pH 6.0 under any one of the lights such as blue light, red light, fluorescent light;
• and
• harvesting the cultured adventitious root washing the cultured adventitious root with tap water and then treating it with 1.0-10.0 mg/L of jasmonic acid or methyl jasmonic acid for 7 days.