Apparatus for separating biological materials
AssigneeSigma-Aldrich Co.
InventorEiichi Sengoku
Life of this patent
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Abstract
A method and apparatus are provided for the separation of biological materials from one another. The device includes a separation column that is in flow communication with a collection chamber in a reservoir that includes a collection container and a lid. The separation column is mounted on the lid. The collection container includes a concentration zone at the bottom and the container is adapted for use in further separation steps including centrifuging. The device includes a connector that is in flow communication with the collection chamber and is adapted for connecting it to a source of vacuum to help induce flow of liquid through the separation column.
Claims16
A separation device for separating biological materials, said device comprising:
- a reservoir comprising a container and a removable lid covering an upper portion of the container, said container defining a liquid collection chamber, said container having an interior bottom surface forming a concentration zone at the bottom of the collection chamber for collecting solids, said concentration zone having a transverse cross sectional area that increases in size from a bottom of the concentration zone to a top of the concentration zone;
- a first connector in fluid flow communication with the collection chamber and adapted for connection to a vacuum source;
- a separation column having an outlet in fluid flow communication with the collection chamber, said separation column containing packing material operable to separate mixed biological materials, said separation column also having an inlet whereby in operation, liquid containing biological materials to be separated is introduced into the column thru the inlet with a portion of the biological material being retained in the column and a portion of the biological materials passing thru the column with the liquid and out the outlet for collection in the collection chamber.
A separation device for separating materials suspended in liquid, said device comprising:
- a reservoir comprising a container and a removable lid covering an upper portion of the container, said container and lid defining a liquid collection chamber having a bottom;
- a connector mounted on said lid and being in fluid flow communication with the collection chamber and adapted for connection to a vacuum source, said connector having an inlet end in said collection chamber and positioned at a first elevation relative to the bottom of the collection chamber;
- a fitting mounted on said lid and being in fluid flow communication with the collection chamber, said fitting having an outlet end in said collection chamber positioned at a second elevation relative to the bottom of the collection chamber, said second elevation being higher than said first elevation by a distance X;
- a separation column mounted on said fitting and in fluid flow communication with the collection chamber, said separation column having an inlet and an outlet whereby in operation, liquid containing materials to be separated is introduced into the column thru the column inlet with a portion of the materials being retained in the column and a portion of the materials passing thru the column with the liquid and out the column outlet for collection in the collection chamber.
Dependencies are inferred from the claim text.