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US 6,713,288 B1Granted
Whole cell catalysts
AssigneesUniversity of Stuttgart, Degussa AG
InventorsJosef Altenbuchner, Andreas Bommarius, Ralf Mattes, Christoph Syldatk, Wilhelm Tischer, Anja Wiese, Burkard Wilms
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Abstract
A microorganism is described which is transformed with DNAs which encode a hydantoinnase, a racemase, and a carbamoylase. As a result, the microorganism is able to degrade hydantoins directly to amino acids. A process for the production of the microorganism and a process for producing amino acids with the microorganism is also described.
Claims56
A microorganism which
- (A) is transformed with DNAs encoding (i) a hydantoinase, (ii) a hydantoin racemase, and (iii) a D- or L-specific carbamoylase, and
- (B) converts 5-monosubstituted hydantoins to L- or D-amino acids,
- wherein the DNAs encoding the hydantoinase, the hydantoin racemase, and the D- or L-specific carbamoylase are overexpressed in the microorganism at a rate which does not result in accumulation of intermediates in the conversion of 5-monosubstituted hydantoins to L- or D-amino acids, and
- wherein the DNAs encoding the hydantoinase, the hydantoin racemase, and the D- or L-specific carbamoylase are obtained from DSM 3747.
1 more element
A microorganism which
- (A) is transformed with DNAs encoding (i) a hydantoinase, (ii) a hydantoin racemase, and (iii) a D- or L-specific carbamoylase, and
- (B) converts 5-monosubstituted hydantoins to L- or D-amino acids,
- wherein the DNAs encoding the hydantoinase, the hydantoin racemase, and the D- or L-specific carbamoylase are overexpressed in a microorganism at a rate which does not result in accumulation of intermediates in the conversion of 5-monosubstituted hydantoins to L- or D-amino acids, and wherein
- the DNA encoding the hydantoinase encodes an amino acid sequence comprising SEQ ID NO:
- 9,
- the DNA encoding the hydantoin racemase encodes an amino acid sequence comprising SEQ ID NO:
- 11,
- the DNA encoding the D- or L-specific carbamoylase encodes an amino acid sequence comprising SEQ ID NO:
- 7.
6 more elements
A microorganism which
- (A) is transformed with DNAs encoding (i) a hydantoinase, (ii) a hydantoin racemase, and (iii) a D- or L-specific carbamoylase, and
- (B) converts 5-monosubstituted hydantoins to L- or D-amino acids,
- wherein the DNAs encoding the hydantoinase, the hydantoin racemase, and the D- or L-specific carbamoylase are overexpressed in a microorganism at a rate which does not result in accumulation of intermediates in the conversion of 5-monosubstituted hydantoins to L- or D-amino acids, and wherein
- the DNA encoding the hydantoinase encodes an amino acid sequence consisting of SEQ ID NO:
- 9,
- the DNA encoding the hydantoin racemase encodes an amino acid sequence consisting of SEQ ID NO:
- 11,
- the DNA encoding the D- or L-specific carbamoylase encodes an amino acid sequence consisting of SEQ ID NO:
- 7.
6 more elements
Dependencies are inferred from the claim text.