Enzymatic synthesis of L-fucose and L-fucose analogs

Abstract

Fucose and fucose analogs are synthesized enzymatically in a three step synthetic protocol. In the first step, L-fuculose-1-phosphate of analog thereof is produced by means of an aldolase catalyzed aldol addition reaction. In the second step, the fuculose-1-phosphate or analog thereof is dephosphorylated to form L-fuculose or an analog thereof using acid dephosphorylase as a catalyst. In the third step, the L-fuculose or analog thereof is converted to L-fucose or an analog thereof using L-fucose isomerase as a catalyst. The synthesis may be a one pot reaction involving the addition substrates and each of the above enzymes to a single reaction vessel. Alternatively, the synthesis may be carried out with purification steps after each reaction.

Dependencies are inferred from the claim text.