Bacterial superantigen vaccines
AssigneeThe United States of America as represented by the Secretary of the Army
InventorsRobert G. Ulrich, Mark A. Olson, Sina Bavari
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Abstract
The present invention relates to genetically attenuated superantigen toxin vaccines altered such that superantigen attributes are absent, however the superantigen is effectively recognized and an appropriate immune response is produced. The attenuated superantigen toxins are shown to protect animals against challenge with wild type toxin. Methods of producing and using the altered superantigen toxins are described.
Claims30
An isolated and purified superantigen toxin DNA fragment encoding Staphylococcal enterotoxin B (SEB) in which at least one amino acid selected from the group consisting of amino acid positions 40-50, 62-72, 84-94 and 110-120 of SEB, and at least one amino acid of amino acid positions 18-28, 55-65, 86-96, 89-99, and 205-215 of SEB have been altered such that binding of said encoded SEB to the MHC class II receptor and T cell antigen receptor is altered.
An isolated and purified superantigen toxin DNA fragment encoding Staphylococcal enterotoxin B (SEB), wherein said superantigen toxin is Staphylococcal enterotoxin B having the sequence of SEQ ID NO:5.
An isolated and purified superantigen toxin DNA fragment encoding Staphylococcal enterotoxin B (SEB), wherein said superantigen toxin is Staphylococcal enterotoxin B having the sequence of SEQ ID NO:7.
An isolated and purified DNA fragment superantigen toxin DNA fragment encoding Staphylococcal enterotoxin B (SEB), wherein said superantigen toxin is Staphylococcal enterotoxin B having the sequence of SEQ ID NO:9.
An isolated and purified DNA fragment superantigen toxin DNA fragment encoding Staphylococcal enterotoxin B (SEB), wherein said fragment encodes the amino acid sequence of SEQ ID NO:6.
An isolated and purified DNA fragment superantigen toxin DNA fragment encoding Staphylococcal enterotoxin B (SEB), wherein said fragment encodes the amino acid sequence of SEQ ID NO:8.
An isolated and purified DNA fragment superantigen toxin DNA fragment encoding Staphylococcal enterotoxin B (SEB), wherein said fragment encodes the amino acid sequence of SEQ ID NO:10.
A recombinant DNA construct comprising a vector containing an isolated and purified superantigen toxin DNA fragment encoding Staphylococcal enterotoxin B (SEB), wherein said DNA fragment has the sequence according to sequence of SEQ ID NO:5.
A recombinant DNA construct comprising a vector containing an isolated and purified superantigen toxin DNA fragment encoding Staphylococcal enterotoxin B (SEB), wherein said DNA fragment has the sequence according to sequence of SEQ ID NO:7.
A recombinant DNA construct comprising a vector containing an isolated and purified superantigen toxin DNA fragment encoding Staphylococcal enterotoxin B (SEB), wherein said DNA fragment has the sequence according to sequence of SEQ ID NO:9.
Dependencies are inferred from the claim text.