Preparing membrane receptors from extracellular baculoviruses
AssigneesValorisation-Recherche, Limited Partnership, Centre National De La Recherche Scientifique
InventorsMichel Bouvier, Thomas Loisel, Stefano Marullo, Pierre Boulanger, Arthur Donny Strosberg
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Abstract
Method for producing membrane receptors based on a baculovirus/insect cell system. The invention further includes the receptors produced by the method described herein, a model for studying the properties of these membrane receptors and an assay to screen molecules that are active on these membrane receptors.
Claims5
Isolated baculovirus viral particles obtained by a method of producing a recombinant membrane receptor protein comprising:
- introducing a baculovirus expression vector encoding said membrane receptor protein into an insect host cell;
- culturing the resultant insect host cell for a sufficient time to permit expression of said membrane receptor protein and baculovirus viral particles;
- separating the cells from the baculovirus viral particles;
- and
- isolating the baculovirus viral particles.
The recombinant baculovirus viral particles of claim 4, wherein at least 90% of said membrane receptor protein is in active form.
Dependencies are inferred from the claim text.