Method for determining alkaline phosphatase
AssigneeRoche Diagnostics GmbH
InventorsRalph Weisheit, Wolfgang Treiber
Life of this patent
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Abstract
The invention concerns a method for the determination of alkaline phosphatase in a sample by optical measurement which is characterized in that a main measurement wavelength of 450±10 nm in combination with the rate blank procedure is used to eliminate haemoglobin interference, a method for eliminating interference by free haemoglobin or blood substitutes and the use of the combination of a main measurement wavelength with the rate blank procedure to eliminate interference by free haemoglobin or blood substitutes in the determination of alkaline phosphatase.
Claims16
A method of eliminating interference by hemoglobin in the determination of alkaline phosphatase in a sample, comprising:
- determination a first optical measurement of said sample at 450±10 nm;
- adding 4-nitrophenyl phosphate to said sample;
- determining a second optical measurement of said sample at 450±10 nm;
- and
- correcting the second optical measurement with the first optical measurement.
A method of determining a level of alkaline phosphatase in a sample containing 4-nitrophenyl phosphate, the method comprising:
- determining an optical measurement of said sample at 450±10 nm;
- and
- correcting the optical measurement by combining the optical measurement with a correction factor that represents a correlation between a degree of hemolysis of said sample and a level of interference due to hemoglobin present in said sample.
A method of determining a level of alkaline phosphatase in a sample, comprising:
- measuring a first change in absorbance of said sample at 450±10 nm;
- adding 4-nitrophenyl phosphate to said sample;
- measuring a second change in absorbance of said sample at 450±10 nm;
- and
- correcting the first change in absorbance with the second change in absorbance.
Dependencies are inferred from the claim text.