High expression and production of high specific activity recombinant S-adenosyl homocysteinase (SAHH) and improved assays for S-adenosylmethionine (SAM)
AssigneeAntiCancer, Inc.
InventorsMingxu Xu, Qinghong Han
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Abstract
The invention provides novel methods relating to SAM detection and production as well as a novel SAHH enzymatic activity for use in such methods. Additional methods, compositions, and kits relating to the novel SAHH are also provided.
Claims8
A method to assess therapeutic levels of S-adenosylmethionine (SAM) in a biological fluid sample which method comprises
- providing said sample with an effective amount of glycine N-methyltransferase (GMT), an effective amount of a S-adenosyl homocysteine hydrolase (SAHH) or His•SAHH (N-terminal histidine tagged SAHH), and glycine;
- and
- measuring one or more reaction products in said sample wherein the level(s) of said one or more reaction products is directly proportional to the level of SAM in the sample.
A kit for assaying a sample containing S-adenosylmethionine (SAM), the kit comprising S-adenosyl homocysteine hydrolase SAHH or His•SAHH (N-terminal histidine tagged SAHH), N-methyltransferase (GMT), glycine and instructions for use.
A method for determining S-adenosylmethionine SAM concentration comprising:
- a biological sample containing SAM;
- and
- an effective amount of glycine N-methyltransferase (GMT), glycine, and S-adenosyl homocysteine hydrolase (SAHH) or N-terminal histadine tagged SAHH (His•SAHH),
- wherein SAHH or His•SAHH activity results in a product that is capable of being measured to determine the amount of SAM in the sample.
Dependencies are inferred from the claim text.