Attachment of biomolecules to hydrophobic surfaces
AssigneeThe United States of America as represented by the Secretary of the Navy
InventorsGeorge P. Anderson, J. Matthew Mauro
Life of this patent
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Abstract
A recombinant protein that is a fusion protein containing a protein binding domain and a domain that is post-translationally modified to become lipid-tagged. This molecule has both a domain with highly specific binding capabilities and a domain which is very hydrophobic in nature. This type of molecule can be used to coat hydrophobic surfaces such as polystyrene easily and effectively for use in solid phase binding assays.
Claims12
A bioactive surface comprising a solid substrate coated with a recombinant protein comprising a fusion protein containing a protein binding domain and a domain that is post-translationally modified to become lipid-tagged;
- wherein the lipid-tagged domain is adsorbed directly to the solid substrate.
A method for solid phase immunoassay comprising the steps of:
- providing a solid substrate coated with a recombinant protein comprising a fusion protein containing a protein binding domain and a domain that is post-translationally modified to become lipid-tagged, the lipid-tagged domain being adsorbed directly to the solid substrate;
- incubating the substrate with antibodies to a target analyte and removing unbound antibody after said incubation;
- incubating the substrate with a sample suspected of containing the target analyte;
- adding a labeled antibody and allowing said labeled antibody to bind to the substrate;
- and
- measuring the amount of the labeled antibody bound to the substrate and correlating the amount of the labeled antibody bound to substrate with the amount of analyte in the sample.
A method for preparing a bioactive surface comprising coating a solid substrate with at least one recombinant protein comprising a fusion protein that contains a bioactive domain and a domain that is post-translationally modified to become lipid-tagged;
- wherein the lipid-tagged domain is adsorbed directly to the substrate.
Dependencies are inferred from the claim text.