DNA chip, PNA chip, and their preparation methods
AssigneeFuji Photo Film Co., Ltd.
InventorsYoshihiko Makino, Yoshihiko Abe, Makoto Takagi, Shigeori Takenaka, Kenichi Yamashita, Masashi Ogawa
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Abstract
A DNA chip (or PNA chip) composed of a solid carrier and plural DNA fragments (or PNA fragments) fixed onto is the solid carrier at each one end, wherein a plurality of short chain spacer molecules having a hydrophilic moiety at each one end are fixed at each another end onto a surface of the solid carrier having no DNA fragments (or no PNA fragments) on its surface is effective for high sensitive quantitative analysis of a nucleic acid fragment complementary to the DNA fragment (or PNA fragment).
Claims5
A DNA chip comprising a solid carrier and a plurality of DNA fragments having one end fixed onto the solid carrier, wherein a plurality of short chain spacer molecules selected from the group consisting of 2-mercaptoethanol, 3-mercaptopropanol and N,N′-di(3-hydroxy-n-propyl)limidazole-2-thione are fixed through a sulfur atom of the molecule onto a surface area of the solid carrier having no DNA fragments thereon.
A process for preparing a DNA chip comprising a solid carrier and a plurality of DNA fragments fixed onto the solid carrier at each one end, wherein a plurality of short chain spacer molecules having a hydrophilic moiety at each one end are fixed through an opposite end thereof onto a surface area of the solid carrier having no DNA fragments thereon which comprises the steps of applying onto a solid carrier an aqueous solution of a plurality of DNA fragments dissolved or dispersed in an aqueous medium to fix the DNA fragments onto the solid carrier, and applying onto a surface area of the solid carrier having no DNA fragments thereon an aqueous solution of short chain spacer molecules each having at one end a hydrophilic moiety and at an opposite end thereof a moiety reactive to fix to the solid carrier.
Dependencies are inferred from the claim text.