Identification of human allergens and T-lymphocyte antigens in vitro
AssigneeThe Research Foundation of State University of New York
InventorRichard S. Kalish
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Abstract
The invention provides a method for screening a test compound for the ability of the test compound to induce a response from human naive T-cells. The method comprises admixing human naive T cells, macrophages/monocytes, immortalized B cells lacking class I and class II major histocompatibility antigens, and a test compound; and determining whether the test compound induces a response from the human naive T cells. The invention further provides a method for primary in vitro sensitization of human naive T-cells. The method comprises admixing human naive T cells, macrophages/monocytes, immortalized B cells lacking class I and class II major histocompatibility antigens, and an antigen.
Claims46
A method for screening a test compound for the ability of the test compound to induce a response from human naive T-cells, the method comprising:
- obtaining a sample of human blood, wherein the sample of human blood contains human naive T cells and macrophages/monocytes;
- admixing the sample of human blood with immortalized B cells lacking class I and class II major histocompatibility antigens and with a test compound, wherein the B cells act as co-stimulatory molecules;
- and
- determining whether the test compound induces a response from the human naive T cells.
A method for primary in vitro sensitization of human naive T-cells, the method comprising:
- obtaining a sample of human blood, wherein the sample of human blood contains human naive T cells and macrophages/monocytes;
- and
- admixing the sample of human blood with immortalized B cells lacking class I and class II major histocompatibility antigens and with an antigen, wherein the B cells act as co-stimulatory molecules.
Dependencies are inferred from the claim text.