Immunologically active polypeptides with altered toxicity useful for the preparation of an antipertussis vaccine
AssigneeChiron Corporation
InventorsMariagrazia Pizza, Antonella Bartoloni, Rino Rappuoli
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Abstract
Immunologically active polypeptides with no or reduced toxicity useful for the preparation of an antipertussis vaccine. Method for the preparation of said polypeptides which comprises, cultivating, a microorganism transformed with a hybrid plasmid including the gene/s which codes for at least one of said polypeptides in a suitable medium and recovering the desired polypeptide from the cells or from the culture medium.
Claims8
An immunologically active modified pertussis toxin comprising the S1, S2, S3, S4 and S5 subunits of pertussis toxin having the same arrangement and configuration as that present in the natural pertussis toxin wherein said S1 subunit is modified by the substitution of glutamic acid at position 129 with glycine and the substitution of arginine at position 9 with another natural amino acid.
Dependencies are inferred from the claim text.