Broad-spectrum δ-endotoxins
AssigneeMonsanto Technology LLC
InventorsThomas Malvar, Komarlingam Sukavaneaswaran Mohan, Sakuntala Sivasupramaniam
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Abstract
Disclosed are novel synthetically-modified B. thuringiensis chimeric crystal proteins having improved insecticidal activity and broader insect host range against coleopteran, dipteran and lepidopteran insects. Also disclosed are the nucleic acid segments encoding these novel peptides. Methods of making and using these genes and proteins are disclosed as well as methods for the recombinant expression, and transformation of suitable host cells. Transformed host cells and transgenic plants expressing the modified endotoxin are also aspects of the invention.
Claims6
A modified, insecticidal Bacillus thuringiensis hybrid crystal protein comprising domains I and II of a first native B. thuringiensis delta-endotoxin Cry1A protein, domain III of a second native B. thurintiensis delta-endotoxin Cry1F protein and all or a portion of a protoxin segment of a native delta endotoxin Cry1A, comprising an amino acid sequence at least 90% identical to SEQ ID NO:34.
Dependencies are inferred from the claim text.