Combined preparation for the treatment of neoplasic diseases or of infectious diseases

A process for preparing monocyte derived cells and chemotherapy drugs in the form of a kit for simultaneous, separate, or sequential use for the treatment of cancer or infectious diseases in a patient, comprising the following steps:

• 1) recovering blood derived mononuclear cells directly from blood apheresis or from blood bag collection, followed if necessary by centrifugation, to eliminate the red blood cells granulocytes and platelets, and collection of peripheral blood leukocytes;
• 2) washing peripheral blood leukocytes obtained at the preceding steps by centrifugation (to remove 90% of platelets, red blood cells and debris) to obtain mononuclear cells;
• 3) resuspending the total mononuclear cells (monocytes+lymphocytes) obtained at the preceding step in culture medium (RPMI or IMDM type) at 106 to 2.107 cells/ml, completed by at least one of cytokines and autologous serum, and culture at 37° C. under O2/CO2 atmosphere in hydrophobic gas permeable bags, to obtain monocyte derived cells and contaminating lymphocytes;
• and
• 4) assembling said monocvte derived cells in a kit with chemotherapy drugs.