Growing cells in a reservoir formed of a flexible sterile plastic liner

A method for culturing one or more cell or microorganism, comprising the steps of:

• providing a disposable first flexible sterilized plastic liner forming a reservoir having an opening;
• attaching the first liner to a closure to close the opening;
• introducing into the reservoir a cell suspension comprising culture medium and one or more cells or microorganisms;
• culturing the cell or microorganism in the reservoir;
• detaching the first liner from the closure after culturing the cell or microorganism;
• and
• attaching a second flexible sterilized plastic liner to the closure after detaching the first liner.

A method for culturing cells and/or a microorganism, comprising the steps of:

• providing a first flexible sterile plastic lining forming a reservoir having a first opening:
• introducing a culture media into the first reservoir;
• introducing cells or a microorganism into the first reservoir;
• closing the first opening so that the first opening is substantially closed while maintaining a port for fluid transfer into the first reservoir;
• providing a second reservoir having a culture media;
• circulating the culture media between the first reservoir and the second reservoir through the port in the first opening.