Rapid growing microorganisms for biotechnology applications
AssigneeInvitrogen Corporation
InventorsFredric R. Bloom, Brian J. Schmidt, Jhy-Jhu Lin
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Abstract
The present invention provides novel rapidly growing microorganisms and methods for their use in cloning or subcloning nucleic acid molecules. The rapid growing microorganisms of the present invention form colonies more rapidly than microorganisms typically used in molecular biology and thus provide a significant improvement in in vitro cloning methods used extensively in molecular biology. The invention also relates to kits and compositions used in the methods of the invention.
Claims91
An isolated E. coli lacking endogenous plasmids and having a growth rate that is at least 5% greater than the growth rate of at least one microorganism selected from the group consisting of E. coli MM294, DH5α and DH10B.
A composition comprising E. coli, wherein said E. coli lack endogenous plasmids and have a growth rate that is at least 5% greater than the growth rate of at least one microorganism selected from the group consisting of E. coli MM294, DH5α and DH10B.
A kit for cloning comprising a container containing E. coli lacking endogenous plasmids and having a growth rate that is at least 5% greater than the growth rate of at least one microorganism selected from the group consisting of E. coli MM294, DH5α and DH10B.
An E. coli having deposit number NRRL B-30143 and derivatives thereof.
An E. coli having deposit number NRRL B-30144 and derivatives thereof.
An E. coli having a growth rate that is at least 5% greater than the growth rate of at least one microorganism selected from the group consisting of E. coli MM294, DH5α and DH10B, wherein said E. coli has been made competent.
An E. coli having a growth rate that is at least 5% greater than the growth rate of at least one microorganism selected from the group consisting of NRRL B-30143, and NRRL B-30144, wherein said E. coli has been made competent.
A method of producing E. coli for cloning, comprising:
- (a) obtaining E. coli having endogenous plasmids and having a growth rate that is at least 50% greater than the growth rate of at least one microorganism selected from the group consisting of E. coli MM294, DH5α and DH10B;
- and
- (b) curing said E. coli of endogenous plasmids.
A method of making competent E. coli, comprising:
- (a) obtaining E. coli having a growth rate that is at least 5% greater than the growth rate of at least one microorganism selected from the group consisting of E. coli MM294, DH5α and DH10B;
- and
- (b) treating said E. coli to make it competent.
A method of transforming E. coli, comprising:
- (a) obtaining competent E. coli;
- (b) incubating said E. coli in the presence of one or more vectors under conditions which cause said one or more vectors to be taken up by said E. coli;
- and
- (c) culturing said E. coli;
- wherein said E. coli are E. coli having a growth rate that is at least 5% greater than the growth rate of at least one microorganism selected from the group consisting of E. coli MM294, DH5α and DH10B.
A method of cloning, comprising:
- (a) obtaining competent E. coli;
- (b) transforming said competent E. coli with at least one vector;
- (c) selecting transformed E. coli containing said at least one vector;
- and
- (d) culturing said transformed E. coli;
- wherein said E. coli are E. coli having a growth rate that is at least 5% greater than the growth rate of at least one microorganism selected from the group consisting of E. coli MM294, DH5α and DH10B.
A method of producing a protein or peptide, comprising:
- (a) obtaining competent E. coli;
- (b) transforming into said competent E. coli a vector containing a gene encoding a protein or peptide;
- and
- (c) culturing said transformed E. coli under conditions that cause said transformed E. coli to produce said protein or peptide;
- wherein said E. coli arc E. coli having a growth rate that is at least 5% greater than the growth rate of at least one microorganism selected from the group consisting of E. coli MM294. DH5α and DH10B.
Dependencies are inferred from the claim text.