Methods of diagnosing and treating hepatic cell proliferative disorders
AssigneeThe Johns Hopkins University School of Medicine
InventorsWilliam G. Nelson, Xiaohui Lin, Julia C. Tchou, Jila Bakker
Life of this patent
Estimating the term…
Abstract
Provided are methods and compositions useful for the diagnosis, prognosis and treatment of hepatic cellular proliferative disorders. The methods include the modulation or analysis of hypemethylated glutathione-S-transferase nuleic acid sequence in hepatic samples and biological fluids.
Claims35
A method for detecting liver cancer in a human, comprising detecting hypermethylation of a CpG-containing promoter region at about nucleotide positions −539 to −239 or −195 to +35 of a glutathione-S-transferase P1 (GSTP1) gene transcription start site in a sample comprising nucleic acids from a hepatic tissue specimen, bile, or blood, wherein a hypermethylation of the CpG-containing promoter region is indicative of liver cancer.
A method for detecting liver cancer in a human, comprising contacting a target cellular component containing a nucleic acid with a reagent which reacts with a CpG-containing promoter region at about nucleotide positions −539 to −239 or −195 to +35 of a glutathione-S-transferase P1 (GSTP1) gene transcription start site and detecting hypermethylation of the CpG-containing promoter region, wherein hypermethylation of the promoter region is indicative of liver cancer.
Dependencies are inferred from the claim text.