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US 6,706,474 B1Granted
Nucleic acid enzyme biosensors for ions
AssigneeThe Board of Trustees of the University of Illinois
InventorsYi Lu, Jing Li
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Abstract
A method of detecting the presence of an ion includes contacting a nucleic acid enzyme with a sample suspected of containing the ion, where the enzyme contains a ribonucleotide and is dependent on the ion to produce a product from a substrate, and measuring an amount of the product produced. The ion is Pb2+, and is in the presence of other ions.
Claims45
A method of detecting the presence of an ion, comprising:
- (a) contacting a nucleic acid enzyme, wherein the enzyme comprises a ribonucleotide and is dependent on the ion to produce a product from a substrate, with a sample suspected of containing the ion;
- and
- (b) measuring an amount of the product produced;
- wherein the ion is in the presence of other ions, and the ion is Pb2+;
- wherein the substrate comprises a fluorophore and the enzyme comprises a quencher of the fluorophore, or the enzyme comprises a fluorophore and the substrate comprises a quencher of the fluorophore.
2 more elements
The method of claim 6, wherein a 3′-end of the enzyme comprises the quencher for the fluorophore.
A method of determining the concentration of an ion in a sample, comprising:
- (a) contacting a nucleic acid enzyme, wherein the, enzyme comprises a ribonucleotide and is dependent on the ion to produce a product from a substrate, with the sample containing an unknown concentration of the ion;
- (b) measuring an amount of the product produced;
- and
- (c) comparing the measurement obtained in (b) with that of a standard curve created using known concentrations of the ion;
- wherein the ion is in the presence of other ions;
- the ion is Pb2+;
- and
- wherein the substrate comprises a fluoromhore and the enzyme comprises a quencher of the fluorophore, or the enzyme comprises a fluorophore and the substrate comprises a quencher of the fluorophore.
5 more elements
Dependencies are inferred from the claim text.