US 6,706,473 B1 · Systems and devices for photoelectrophoretic transport and hybridization of oligonucleotides

Abstract

A platform for photoelectrophoretic transport and electronic hybridization of fluorescence labeled DNA oligonucleotides in a low conductivity electrolyte is described. A chemically stabilized semiconductor photodiode or photoconductor surface is coated with a streptavidin-agarose permeation layer. Micro-illumination of the surface generates photo-electrochemical currents that are used to electrophoretically transport and attach capture strands, preferably biotinylated DNA, to arbitrarily selected locations. The same process is then used to transport and electronically hybridize fluorescence labeled DNA target strands to the previously attached capture strands. Signal detection is accomplished either by a fluorescence scanner or a CCD camera. This represents a flexible electronic DNA assay platform that need not rely on pre-patterned microelectronic arrays.

Claims49

Dependent claims

A device for effecting the photoelectric transport of charged materials in a liquid environment, comprising:

a substrate having a first face and a second face, the substrate capable of generating a photocurrent;
a conductor contacting at least a portion of the first face of the substrates
a permeation layer supported on the second face, wherein the permeation layer comprises agarose;
attachment entities coupled to the permeation layer;
and
a light source disposed to illuminate at least a portion of the substrate.

3 more elements

Dependencies are inferred from the claim text.

Facts

Application

09/489,855Filed 24 Jan 2000 · continuation in part

Priority

6 Dec 1996

Granted

16 Mar 2004

Term ends

Not estimatedThe source records don’t support an estimate.

Classification63 codes

G06N 3/061
Physical realisation, i.e. hardware implementation of neural networks, neurons or parts of neurons: using biological neurons, e.g. biological…
B01J 19/0046
Chemical, physical or physico-chemical processes in general; Their relevant apparatus: sequential or parallel reactions, e.g. for the synthesis of…
B82B 3/00
Manufacture or treatment of nanostructures by manipulation of individual atoms or molecules
B82Y 5/00
Nanobiotechnology or nanomedicine

Family

Application08/760,933Parent of this continuation-in-part · filed 6 Dec 1996

Application09/436,311Parent of this continuation-in-part · filed 8 Nov 1999

US 6,569,382 B1Parent of this continuation-in-part

US 6,652,808 B1Parent of this continuation-in-part

US 6,706,473 B1This patent

From the US filing history. Foreign family members aren’t listed.

Systems and devices for photoelectrophoretic transport and hybridization of oligonucleotides

Life of this patent

Systems and devices for photoelectrophoretic transport and hybridization of oligonucleotides

Life of this patent

Abstract

Claims49