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US 6,700,037 B2Granted
Method of cloning porcine animals
AssigneeInfigen, Inc.
InventorsPhilip Damiani, Jeffrey M. Betthauser, Erik J. Forsberg, Michael D. Bishop
Life of this patent
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Abstract
The present invention relates to materials and methods for cloning porcine animals. The invention relates in part to totipotent cells useful for cloning porcine animals, porcine embryos produced from such cells by employing nuclear transfer techniques, and porcine animals that arise from such cells and embryos.
Claims47
A method for preparing a porcine embryo, comprising:
- (a) culturing one or more nonembryonic porcine cells in a medium comprising one or more components selected from the group consisting of LIF, FGF and stem cell factor to obtain one or more cultured cells;
- (b) translocating one of said cultured cells, or a nucleus thereof, into an enucleated porcine oocyte to establish a nuclear transfer oocyte;
- and
- (c) activating said nuclear transfer oocyte to establish said porcine embryo.
1 more element
A method for preparing a cloned porcine embryo, comprising:
- (a) translocating a cultured nonembryonic porcine cell, or a nucleus thereof, into an enucleated porcine oocyte to establish a nuclear transfer oocyte, wherein said porcine oocyte is a sow oocyte;
- and
- (b) activating said nuclear transfer oocyte to establish said porcine embryo.
A method for preparing a porcine embryo, comprising:
- (a) culturing one or more nonembryonic porcine cells in a medium comprising between 10 mM and 100 mM glucose;
- (b) translocating one of said cultured cells, or a nucleus thereof, into an enucleated porcine oocyte to establish a nuclear transfer oocyte;
- and
- (c) activating said nuclear transfer oocyte to establish said porcine embryo.
1 more element
A method for preparing a porcine animal, comprising:
- a) translocating a cultured nonembryonic porcine cell[s], or a nucleus thereof, into an enucleated porcine oocyte to establish a nuclear transfer oocyte;
- b) activating said nuclear transfer oocyte to establish said porcine embryo;
- and
- c) transferring said porcine embryo into a recipient female to produce said porcine animal, wherein said porcine embryo and said recipient female are asynchronous.
1 more element
Dependencies are inferred from the claim text.