Methods and apparatus for perfusion of isolated tissue structure

A method of treatment of a tissue structure in a patient, the tissue structure being coupled to an artery through which blood flows from the patient's circulatory system to the tissue structure and to a vein through which blood flows away from the tissue structure to the circulatory system, the method comprising:

• endovascularly positioning a first catheter in the artery;
• endovascularly positioning a second catheter in the vein;
• occluding the artery around the first catheter;
• occluding the vein around the second catheter;
• delivering a perfusate comprising a therapeutic agent and an oxygen carrier to the tissue structure through at least one of the first and second catheters while the artery and vein remain occluded, the tissue structure being an organ selected from the group consisting of the brain, at least one breast, at least one lung, at least one kidney, the liver, the spleen, the stomach, the intestines, the pancreas, the colon, the bladder, the thyroid gland, and the prostate;
• collecting the perfusate through the other of the at least one of the first and second catheters after the perfusate has perfused at least a portion of the tissue structure;
• extracorporeally pumping the perfusate back to the at least one of the first and second catheters;
• and
• extracorporeally oxygenating the oxygen carrier.