Methods and devices for processing blood samples
OwnerJames Murto, Michael Salvatiinventors
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Abstract
Approaches are described for separating plasma from whole blood samples and include the use of magnetically attractable particles associated with an agglutinating agent. The magnetically attractable particles bind the cellular components in a whole blood sample. Application of a magnetic field gradient to a container with the blood sample and the magnetically attractable particles draws the particles to the surface of the container near the source of the magnetic field gradient. The plasma can be removed and stored or used for monitoring or detecting analytes in the plasma.
Claims53
A method for processing blood comprising:
- providing blood comprising cellular components and plasma, said plasma comprising positively and negatively charged analytes;
- providing a magnetically attractable particle associated with an agglutinating agent, said agglutinating agent capable of selectively binding to said cellular components and not to said analytes;
- contacting said blood with said magnetically attractable particle associated with said agglutinating agent to form a complex;
- and
- subjecting said contacted blood to a magnetic field to separate said complex from said plasma.
A method for processing blood comprising:
- providing blood comprising cellular components and plasma;
- providing a magnetically attractable particle associated with an agglutinating agent, said agglutinating agent capable of selectively binding to said cellular components;
- contacting said blood with said magnetically attractable particle to form a complex;
- and
- subjecting said contacted blood to a magnetic field to separate said complex from said plasma to result in magnetically-separated plasma, wherein said magnetically-separated plasma is substantially equivalent to centrifugation-derived plasma.
A device for processing blood, said blood comprising cellular components and plasma, said plasma comprising positively and negatively charged analytes, to separate said cellular components from said plasma, said device comprising a container having magnetically attractable particles associated with an agglutinating agent, said agglutinating agent capable of selectively binding to said cellular components and not to said positively and negatively charged analytes, wherein the volume of said magnetically attractable particles in said container is selected such that when a magnetic field is applied to a suspension of said magnetically attractable particles, said magnetically attractable particles accumulate in less than about 5 minutes at a surface of said container near to said magnetic field, and wherein at least about 0.2 micrograms of said agglutinating agent is associated with about 1 milligram of said magnetically attractable particles.
A device for processing blood, said blood comprising cellular components and plasma, said plasma comprising positively and negatively charged analytes, to separate said cellular components from said plasma, said device comprising a container having magnetically attractable particles associated with an agglutinating agent, said agglutinating agent capable of selectively binding to said cellular components and not to said positively and negatively charged analytes, wherein the volume of said magnetically attractable particles in said container is selected such that when a magnetic field is applied to a suspension of said magnetically attractable particles, said magnetically attractable particles accumulate in less than about 5 minutes at a surface of said container near to said magnetic field, and wherein between about 1 microgram and about 5 micrograms of said agglutinating agent is associated with about 1 milligram of said magnetically attractable particles.
A device for processing blood, said blood comprising cellular components and plasma, said plasma comprising positively and negatively charged analytes, to separate said cellular components from said plasma, said device comprising a container having magnetically attractable particles associated with an agglutinating agent, said agglutinating agent capable of selectively binding to said cellular components and not to said positively and negatively charged analytes, wherein the volume of said magnetically attractable particles in said container is selected such that when a magnetic field is applied to a suspension of said magnetically attractable particles, said magnetically attractable particles accumulate in less than about 5 minutes at a surface of said container near to said magnetic field, and wherein said magnetically attractable particles are in a suspension.
A device for processing blood, said blood comprising cellular components and plasma, said plasma comprising positively and negatively charged analytes, to separate said cellular components from said plasma, said device comprising a container having magnetically attractable particles associated with an agglutinating agent, said agglutinating agent capable of selectively binding to said cellular components and not to said positively and negatively charged analytes, wherein the volume of said magnetically attractable particles in said container is selected such that when a magnetic field is applied to a suspension of said magnetically attractable particles, said magnetically attractable particles accumulate in less than about 5 minutes at a surface of said container near to said magnetic field, and wherein said magnetically attractable particles are in a dry form.
A device for processing blood, said blood comprising cellular components and plasma, said plasma comprising positively and negatively charged analytes, to separate said cellular components from said plasma, said device comprising a container having magnetically attractable particles associated with an agglutinating agent, said agglutinating agent capable of selectively binding to said cellular components and not to said positively and negatively charged analytes, wherein the volume of said magnetically attractable particles in said container is selected such that when a magnetic field is applied to a suspension of said magnetically attractable particles, said magnetically attractable particles accumulate in less than about 5 minutes at a surface of said container near to said magnetic field, and wherein said magnetically attractable particles accumulate in less than about 3 minutes.
A device for processing blood, said blood comprising cellular components and plasma, said plasma comprising positively and negatively charged analytes, to separate said cellular components from said plasma, said device comprising a container having magnetically attractable particles associated with an agglutinating agent, said agglutinating agent capable of selectively binding to said cellular components and not to said positively and negatively charged analytes, wherein the volume of said magnetically attractable particles in said container is selected such that when a magnetic field is applied to a suspension of said magnetically attractable particles, said magnetically attractable particles accumulate in less than about 5 minutes at a surface of said container near to said magnetic field, and wherein said container further comprises an anti-coagulant.
A device for processing blood, said blood comprising cellular components and plasma, said plasma comprising positively and negatively charged analytes, to separate said cellular components from said plasma, said device comprising a container having magnetically attractable particles associated with an agglutinating agent, said agglutinating agent capable of selectively binding to said cellular components and not to said positively and negatively charged analytes, wherein the volume of said magnetically attractable particles in said container is selected such that when a magnetic field is applied to a suspension of said magnetically attractable particles, said magnetically attractable particles accumulate in less than about 5 minutes at a surface of said container near to said magnetic field, and wherein said container further comprises a non-hemolytic surfactant.
A device for processing blood, said blood comprising cellular components and plasma, said plasma comprising positively and negatively charged analytes, to separate said cellular components from said plasma, said device comprising a container having magnetically attractable particles associated with an agglutinating agent, said agglutinating agent capable of selectively binding to said cellular components and not to said positively and negatively charged analytes, wherein the volume of said magnetically attractable particles in said container is selected such that when a magnetic field is applied to a suspension of said magnetically attractable particles, said magnetically attractable particles accumulate in less than about 5 minutes at a surface of said container near to said magnetic field, and wherein said volume of said magnetically attractable particles in said container is sufficient to bind at least about 95 percent of red blood cells in blood.
A device for processing blood, said blood comprising cellular components and plasma, said plasma comprising positively and negatively charged analytes, to separate said cellular components from said plasma, said device comprising a container having magnetically attractable particles associated with an agglutinating agent, said agglutinating agent capable of selectively binding to said cellular components and not to said positively and negatively charged analytes, wherein the volume of said magnetically attractable particles in said container is selected such that when a magnetic field is applied to a suspension of said magnetically attractable particles, said magnetically attractable particles accumulate in less than about 5 minutes at a surface of said container near to said magnetic field, and wherein said volume of said magnetically attractable particles in said container is sufficient to bind at least about 99 percent of red blood cells in blood.
A device for processing blood, said blood comprising cellular components and plasma, said plasma comprising positively and negatively charged analytes, to separate said cellular components from said plasma, said device comprising a container having magnetically attractable particles associated with an agglutinating agent, said agglutinating agent capable of selectively binding to said cellular components and not to said positively and negatively charged analytes, wherein the volume of said magnetically attractable particles in said container is selected such that when a magnetic field is applied to a suspension of said magnetically attractable particles, said magnetically attractable particles accumulate in less than about 5 minutes at a surface of said container near to said magnetic field, and further comprising a magnet.
A device for processing blood, said blood comprising cellular components and plasma, said plasma comprising positively and negatively charged analytes, to separate said cellular components from said plasma, said device comprising a container having magnetically attractable particles associated with an agglutinating agent, said agglutinating agent capable of selectively binding to said cellular components and not to said positively and negatively charged analytes, wherein the volume of said magnetically attractable particles in said container is selected such that when a magnetic field is applied to a suspension of said magnetically attractable particles, said magnetically attractable particles accumulate in less than about 5 minutes at a surface of said container near to said magnetic field, and further comprising one or more components for analyzing the presence or absence of an analyte in said plasma.
A reagent to separate cellular components from plasma, said cellular components and said plasma present in blood, said reagent comprising magnetically attractable particles associated with an agglutinating agent, said agglutinating agent capable of selectively binding to said cellular components and not to positively and negatively charged analytes present in said plasma, wherein the volume of said magnetically attractable particles is such that when said magnetically attractable particles are in suspension, said magnetically attractable particles are drawn away from the liquid of said blood in less than about five minutes when a magnetic field is applied, and wherein at least about 0.2 micrograms of said agglutinating agent is associated with about 1 milligram of said magnetically attractable particles.
A reagent to separate cellular components from plasma, said cellular components and said plasma present in blood, said reagent comprising magnetically attractable particles associated with an agglutinating agent, said agglutinating agent capable of selectively binding to said cellular components and not to positively and negatively charged analytes present in said plasma, wherein the volume of said magnetically attractable particles is such that when said magnetically attractable particles are in suspension, said magnetically attractable particles are drawn away from the liquid of said blood in less than about five minutes when a magnetic field is applied, and wherein between about 1 microgram and about 5 micrograms of said agglutinating agent is associated with about 1 milligram of said magnetically attractable particles.
A reagent to separate cellular components from plasma, said cellular components and said plasma present in blood, said reagent comprising magnetically attractable particles associated with an agglutinating agent, said agglutinating agent capable of selectively binding to said cellular components and not to positively and negatively charged analytes present in said plasma, wherein the volume of said magnetically attractable particles is such that when said magnetically attractable particles are in suspension, said magnetically attractable particles are drawn away from the liquid of said blood in less than about five minutes when a magnetic field is applied, and wherein said magnetically attractable particles are in suspension.
A reagent to separate cellular components from plasma, said cellular components and said plasma present in blood, said reagent comprising magnetically attractable particles associated with an agglutinating agent, said agglutinating agent capable of selectively binding to said cellular components and not to positively and negatively charged analytes present in said plasma, wherein the volume of said magnetically attractable particles is such that when said magnetically attractable particles are in suspension, said magnetically attractable particles are drawn away from the liquid of said blood in less than about five minutes when a magnetic field is applied, and wherein said magnetically attractable particles are in a dry form.
Dependencies are inferred from the claim text.