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AssigneePlantTec Biotechnologie GmbH Forshung & Entwicklung
InventorsJens Kossmann, Claus Frohberg
Estimating the term…
Nucleic acid molecules are described which encode enzymes involved in the starch synthesis in plants. These enzymes are starch phosphorylases from maize. The invention further relates to vectors containing such nucleic acid molecules and to host cells transformed with the described nucleic acid molecules, in particular to transformed plant cells and to plants which may be regenerated therefrom and which exhibit an increased or reduced activity of the described proteins.
An isolated nucleic acid molecule comprising a nucleic acid sequence, wherein the nucleic acid sequence is selected from the group consisting of
An isolated nucleic acid molecule comprising a nucleic acid sequence, wherein the nucleic acid sequence is selected from the group consisting of:
Dependencies are inferred from the claim text.
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From the US filing history. Foreign family members aren’t listed.
Glycosyltransferases: hexosyltransferases
Involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine: involving modified carbohydrate or sugar…
Microorganisms or enzymes
Term ended 16 Mar 2018 (estimated)
The present invention relates to nucleic acid molecules encoding a starch phosphorylase from maize. Furthermore, the present invention relates to vectors, bacteria as well as to plant cells transformed with the described nucleic acid molecules and to the plants containing the same. Moreover, methods for the production of transgenic plants are described which, due to the introduction of DNA molecules encoding a starch phosphorylase from maize, synthesize a starch which is modified in its properties.
With respect to the increasing significance which has recently been ascribed to vegetal substances as regenerative sources of raw materials, one of the objects of biotechnological research is to try to adapt vegetal raw materials to the demands of the processing industry. In order to enable the use of regenerative raw materials in as many areas as possible, it is furthermore important to obtain a large variety of substances.
Apart from oils, fats and proteins, polysaccharides constitute the essential regenerative raw materials derived from plants. Apart from cellulose, starch maintains an important position among the polysaccharides, being one of the most significant storage substances in higher plants. Among those, maize is one of the most interesting plants as it is the most important cultivated plant for the production of starch.
FIG. 1 shows a construct for antisense inhibition of a plastidic isoform of starch phosphorylase in maize.
The examples illustrate the invention.
Media and solutions used in the examples:
20 × SSC: 175.3 g NaCl 88.2 g sodium citrate ad 1000 ml with ddH2O pH 7.0 with 10 N NaOH YT 8 g Bacto-Yeast extract 5 g Bacto-Tryptone 5 g NaCl ad 1000 ml with ddH2O Protoplast isolation medium 800 mg 2 (100 ml) Cellulase Onozuka R S (Meiji Seika, Japan) Pectolyase Y 23 40 mg KNO3 200 mg KH2PO4 136 mg K2HPO4 47 mg CaCl2 2H2O 147 mg MgSO4 7H2O 250 mg Bovine serum albumine (BSA) 20 mg Glucose 4000 mg Fructose 4000 mg Sucrose 1000 mg pH 5.8 Osmolarity 660 mosm. Protoplast washing solution 1: like protoplast isolating solution, but without cellulase, pectolyase and BSA