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US 6,686,461 B1Granted
Triphosphate oligonucleotide modification reagents and uses thereof
AssigneeSolulink Bioscience, Inc.
InventorsDavid A. Schwartz, Richard I. Hogrefe
Life of this patent
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Abstract
Hydrazino, oxyamino and carbonyl-based monomers and methods for incorporation into oligonucleotides during enzymatic synthesis are provided. Modified oligonucleotides are provided that incorporate the monomers provided herein. Immobilized oligonucleotides and oligonucleotide conjugates that contain covalent hydrazone or oxime linkages are provided. Methods for preparation of surface bound oligonucleotides are provided. Methods for the preparation of oligonucleotide conjugates are also provided.
Claims9
A compound that has formula (I) or salt thereof:
- P—S—B—M—X (I)
- wherein:
- P is a triphosphate group;
- S is a a deoxyribose;
- B is a pyrimidine nucleobase;
- X is —NHNH2, —ONH2, a protected hydrazino, a protected oxyamino, —CHO or —COMe;
- and
- M is a divalent group selected from the group consisting of:
5 more elements
Dependencies are inferred from the claim text.