Genetic manipulation of spermatogonia
AssigneeTran Xenogen, Inc.
InventorsPaul A. DiTullio, Karl M. Ebert
Life of this patent
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Abstract
The invention features a method of delivering DNA to a spermatogonium by infusing DNA in situ into a testicle of a non-human animal and administering a condition or substance to the testicle to increase uptake of DNA by the spermatogonium.
Claims19
A method of delivering a DNA to a spermatogonium, comprising infusing in situ said DNA into a testicle of a prepubertal non-human mammal and administering an electrical current to said testicle to facilitate uptake of said DNA by said spermatogonium, wherein said DNA is infused into said testicle before production of sperm by meiosis in said testicle.
A method of making a non-human transgenic animal comprising infusing in situ DNA into a testicle of a prepubertal non-human mammal, harvesting sperm cells from said mammal, contacting an ovum with said sperm cells under conditions suitable for fertilization to produce said nonhuman transgenic mammal.
A method of delivering a DNA to a spermatogonium, comprising infusing in situ said DNA into a testicle of a prepubertal non-human mammal and administering a lipid or phospholipid to said testicle to facilitate uptake of said DNA by said spermatogonium, wherein said DNA is infused into said testicle before production of sperm by meiosis in said testicle.
A method of delivering a DNA to a spermatogonium, comprising infusing said DNA directly into a testicle of a prepubertal non-human mammal, wherein said DNA is packaged into a viral vector and is infused into said testicle before production of sperm by meiosis in said testicle.
A method of delivering a naked DNA to a spermatogonium, comprising infusing in situ said naked DNA into a testicle of a prepubertal non-human mammal, wherein said naked DNA is infused into said testicle before production of sperm by meiosis in said testicle.
A method of delivering a DNA to a spermatogonium, comprising infusing in situ said DNA into a testicle of a prepubertal non-human mammal and administering DEAE-dextran to said testicle to facilitate uptake of said DNA by said spermatogonium, wherein said DNA is infused into said testicle before production of sperm by meiosis in said testicle.
A method of delivering a DNA to a spermatogonium, comprising infusing in situ said DNA into a testicle of a non-human mammal and administering an electrical current to said testicle to facilitate uptake of said DNA by said spermatogonium, wherein said DNA is infused into said testicle before production of sperm by meiosis in said testicle and wherein said method does not comprise destruction of spermatogenic cells in said animal prior to infusing said DNA into said testicle.
Dependencies are inferred from the claim text.