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US 6,686,197 B2Granted
Method for producing preparations of mature and immature pancreatic endocrine cells, the cell preparation and its use for treatment of diabetes mellitus
AssigneeBeta-Cell N.V.
InventorDaniel Pipeleers
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Abstract
A method for preparing a preparation of mammalian pancreatic endocrine cells comprising the steps: dissociating intact pancreatic tissue into a cell suspension comprising single cells and cell aggregates; enriching said cell suspension with regard to the content in endocrine cells by separating single cells and cellular aggregates with size <100 μm; and removing contaminating non-endocrine cells by density centrifugation.
Claims21
A method for preparing a preparation of mammalian pancreatic endocrine cells comprising:
- dissociating intact pancreatic tissue into a cell suspension comprising single cells and cellular aggregates;
- removing single cell and cellular aggregates larger than 100 μm from said cell suspension;
- enriching said cell suspension with regard to the content in endocrine cells and cellular aggregates with size ≦100 μm;
- and
- removing contaminating non-endocrine cells by density centrifugation, wherein the resulting preparation of mammalian pancreatic endocrine cells consists essentially of endocrine cells and cellular aggregates having sizes ≦100 μm.
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