Antisense RNA expression strategies effective against Streptococcus thermophilus bacteriophages
AssigneeNorth Carolina State University
InventorsJoseph Miland Sturino, Todd Robert Klaenhammer
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Abstract
Recombinant bacteria containing nucleotide sequences encoding Streptococcus thermophilus phage helicase and primase genes expressed in the antisense orientation and methods of making and using the same are disclosed. Replication of bacteriophage infecting this bacterial cell, can be inhibited or decreased by constitutively expressing, the antisense oligonucleotide encoding for S. thermophilus phage helicase or primase.
Claims20
An isolated nucleic acid encoding an antisense oligonucleotide that binds to an RNA sequence encoded by a Streptococcus thermophilus bacteriophage, which antisense oligonucleotide inhibits the replication of said bacteriophage in a Streptococcus thermophilus cell;
- wherein said RNA is a phage protein mRNA, said phage protein selected from the group consisting of primase and helicase;
- and wherein said phage is selected from the group consisting of cos-type phages and pac-type phages.
A recombinant nucleic acid comprising a nucleic acid encoding an antisense oligonucleotide operatively associated with a promoter, wherein said antisense oligonucleotide binds to an RNA sequence encoded by a Streptococcus thermophilus bacteriophage, which antisense oligonucleotide inhibits the replication of said bacteriophage in a Streptococcus thermophilus cell;
- wherein said RNA is a phage protein mRNA, said phage protein selected from the group consisting of primase and helicase;
- and wherein said phage is selected from the group consisting of cos-type phages and pac-type phages.
A Streptococcus thermophilus cell containing a recombinant nucleic acid, said recombinant nucleic acid comprising a nucleic acid encoding an antisense oligonucleotide operatively associated with a promoter, wherein said antisense oligonucleotide binds to an RNA sequence encoded by a Streptococcus thermophilus bacteriophage, which antisense oligonucleotide inhibits the replication of said bacteriophage in a Streptococcus thermophilus cell;
- wherein said RNA is a phage protein mRNA, said phage protein selected from the group consisting of primase and helicase;
- and wherein said phage is selected from the group consisting of cos-type phages and pac-type phages.
Dependencies are inferred from the claim text.