Method for the production of bacterial toxins
AssigneesBaxter International Inc., Baxter Healthcare S.A.
InventorsMilan S. Blake, John A. Bogdan, Jr., Javier Nazario-Larrieu
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Abstract
Methods and compositions are provided for the enhanced production of bacterial toxins in large-scale cultures. Specifically, methods and compositions for reducing bacterial toxin expression inhibitors are providing including, but not limited to, addition of toxin expression inhibitor binding compounds, culture media having reduced concentrations of toxin inhibitor metabolic precursors and genetically modified toxogenic bacteria lacking enzymes required to metabolize the toxin inhibitor metabolic precursors.
Claims5
A method of producing pertussis toxin, comprising
- cultivating a Bordetella species in a media that comprises one or more salts selected from the group consisting of Ba salts, Sr (II) salts, Pb (II) salts and Ag (II) salts to eliminate or reduce pertussis toxin expression inhibitors;
- and
- isolating the pertussis toxin from the media.
A method of producing a Bordetella toxin, comprising
- cultivating a Bordetella species in a media that comprises one or more salts selected from the group consisting of Ba salts, Sr (II) salts, Pb (II) salts and Ag (II) salts, wherein the salts sequester sulfate ions that inhibit expression of the toxin;
- and
- isolating the Bordetella toxin from the media.
Dependencies are inferred from the claim text.