IP-10/MIG receptor designated CXCR3, nucleic acids, and methods of use therefor

An isolated nucleic acid encoding a protein comprising the amino acid sequence of FIG. 2 (SEQ ID NO:2).

An isolated nucleic acid encoding a human CXC Chemokine Receptor 3 (CXCR3) protein, wherein the amino acid sequence of said CXCR3 protein is a sequence encoded by the nucleic acid of FIG. 1 (SEQ ID NO:1).

A recombinant nucleic acid encoding a fusion protein comprising a human CXC Chemokine Receptor 3 (CXCR3) protein, wherein said CXCR3 protein binds one or more chemokines selected from the group consisting of human IP-10 and human Mig, and wherein said CXCR3 protein is encoded by a nucleic acid which hybridizes to a second nucleic acid selected from the group consisting of the complement of SEQ ID NO:1 and the complement of the open reading frame of SEQ ID NO:1 under high stringency wash conditions of 2×SSC, 0.1% SDS at room temperature for ten minutes followed by two washes in 1×SSC, 0.1% SDS at 65° C. for thirty minutes and a final wash in 0.5×SSC, 0.1% SDS at 65° C. for ten minutes.

A recombinant nucleic acid encoding a fusion protein comprising a human CXC Chemokine Receptor 3 (CXCR3) protein, wherein the amino acid sequence of said CXCR3 protein is a sequence encoded by the nucleic acid of FIG. 1 (SEQ ID NO:1).

A recombinant nucleic acid encoding a fusion protein comprising a human CXC Chemokine Receptor 3 (CXCR3) protein, wherein the amino acid sequence of said CXCR3 protein consists of the amino acid sequence of FIG. 2 (SEQ ID NO:2).

A recombinant nucleic acid construct comprising a nucleic acid encoding a human CXC Chemokine Receptor 3 (CXCR3) protein, wherein said CXCR3 protein binds one or more chemokines selected from the group consisting of human IP-10 and human Mig, and wherein said nucleic acid encoding a human CXCR3 protein hybridizes to a second nucleic acid selected from the group consisting of the complement of SEQ ID NO:1 and the complement of the open reading frame of SEQ ID NO:1 under high stringency wash conditions of 2×SSC, 0.1% SDS at room temperature for ten minutes followed by two washes in 1×SSC, 0.1% SDS at 65° C. for thirty minutes and a final wash in 0.5×SSC, 0.1% SDS at 65° C. for ten minutes.

A recombinant nucleic acid construct comprising a nucleic acid encoding a human CXC Chemokine Receptor 3 (CXCR3) protein, wherein the amino acid sequence of said CXCR3 protein is a sequence encoded by the nucleic acid of FIG. 1 (SEQ ID NO:1).

A recombinant nucleic acid construct comprising a nucleic acid encoding a protein comprising the amino acid sequence of FIG. 2 (SEQ ID NO:2).

A host cell comprising a recombinant nucleic acid encoding a human CXC Chemokine Receptor 3 (CXCR3) protein, wherein said CXCR3 protein binds one or more chemokines selected from the group consisting of human IP-10 and human Mig, wherein said nucleic acid encoding a human CXCR3 protein hybridizes to a second nucleic acid selected from the group consisting of the complement of SEQ ID NO:1 and the complement of the open reading frame of SEQ ID NO:1 under high stringency wash conditions of 2×SSC, 0.1% SDS at room temperature for ten minutes followed by two washes in 1×SSC, 0.1% SDS at 65° C. for thirty minutes and a final wash in 0.5×SSC, 0.1% SDS at 65° C. for ten minutes, and wherein said host cell is not in a transgenic animal.

A host cell comprising a recombinant nucleic acid encoding a human CXC Chemokine Receptor 3 (CXCR3) protein, wherein the amino acid sequence of said CXCR3 protein is a sequence encoded by the nucleic acid of FIG. 1 (SEQ ID NO:1), and wherein said host cell is not in a transgenic animal.

A host cell comprising a recombinant nucleic acid encoding a protein comprising the amino acid sequence of FIG. 2 (SEQ ID NO:2), wherein said host cell is not in a transgenic animal.

A method for producing a human CXC Chemokine Receptor 3 (CXCR3) protein, wherein said protein binds one or more chemokines selected from the group consisting of human IP-10 and human Mig, the method comprising:

• (a) introducing into a host cell a nucleic acid encoding said human CXCR3 protein, wherein said nucleic acid hybridizes to a second nucleic acid selected from the group consisting of the complement of SEQ ID NO:1 and the complement of the open reading frame of SEQ ID NO:1 under high stringency wash conditions of 2×SSC, 0.1% SDS at room temperature for ten minutes followed by two washes in 1×SSC, 0.1% SDS at 65° C. for thirty minutes and a final wash in 0.5×SSC, 0.1% SDS at 65° C. for ten minutes, whereby a recombinant host cell is produced having said nucleic acid operably linked to an expression control sequence;
• and
• (b) maintaining the recombinant host cell produced in step (a) under conditions whereby the nucleic acid is expressed.

An isolated nucleic acid having a sequence that is the complement of a second nucleic acid which encodes a human CXC Chemokine Receptor 3 (CXCR3) protein, wherein said protein binds one or more chemokines selected from the group consisting of human IP-10 and human Mig, and wherein said second nucleic acid hybridizes to a third nucleic acid selected from the group consisting of the complement of SEQ ID NO:1 and the complement of the open reading frame of SEQ ID NO:1 under high stringency wash conditions of 2×SSC, 0.1% SDS at room temperature for ten minutes followed by two washes in 1×SSC, 0.1% SDS at 65° C. for thirty minutes and a final wash in 0.5×SSC, 0.1% SDS at 65° C. for ten minutes.

An isolated nucleic acid that is the complement of SEQ ID NO:1 or the complement of a portion of SEQ ID NO:1 comprising the open reading frame.

An isolated nucleic acid that is the complement of a second nucleic acid which encodes a protein comprising the amino acid sequence of SEQ ID NO:2.

An isolated nucleic acid that is the complement of a second nucleic acid which encodes a human CXC Chemokine Receptor 3 (CXCR3) protein, wherein the amino acid sequence of said CXCR3 protein is a sequence encoded by the nucleic acid of FIG. 1 (SEQ ID NO:1).

A recombinant nucleic acid having a sequence that is the complement of a second nucleic acid which encodes a human CXC Chemokine Receptor 3 (CXCR3) protein, wherein said protein binds one or more chemokines selected from the group consisting of human IP-10 and human Mig, and wherein said second nucleic acid hybridizes to a third nucleic acid selected from the group consisting of the complement of SEQ ID NO:1 and the complement of the open reading frame of SEQ ID NO:1 under high stringency wash conditions of 2×SSC, 0.1% SDS at room temperature for ten minutes followed by two washes in 1×SSC, 0.1% SDS at 65° C. for thirty minutes and a final wash in 0.5×SSC, 0.1% SDS at 65° C. for ten minutes.

An isolated nucleic acid encoding a human CXC Chemokine Receptor 3 (CXCR3) protein, wherein said nucleic acid comprises SEQ ID NO:1 or a portion of SEQ ID NO:1 comprising the open reading frame.