Reagent for the detection of Staphylococcus aureus by agglutination
AssigneeInstitut Pasteur
InventorsJean-Michel Fournier, Alain Boutonnier
Life of this patent
Estimating the term…
Abstract
Reagents and methods for the detection of Staphylococcus aureus are provided. The reagents contain an antibody that binds to a capsular polysaccharide of type 5 of Staphylococcus aureus, and can be used in methods for detection of oxacillin resistant Staphylococcus aureus that escapes detection by agglutination in the presence of fibrinogen and antibodies directed against protein A of Staphylococcus.
Claims21
A Reagent for the detection by agglutination of Staphylococcus aureus, comprising a mixture of (A) particles in suspension to which are bound fibrinogen, (B) particles in suspension to which are bound antibodies, which have affinity for protein A of Staphylococcus, and (C) particles in suspension to which are bound at least one antibody, which binds to a capsular polysaccharide of type 5 of Staphylococcus aureus, wherein said reagent can detect oxacillin resistant Staphylococcus aureus that is not detected by said reagent in the absence of the antibody that binds to the capsular polysaccharide.
A reagent for the detection by agglutination of Staphylococcus aureus comprising a mixture of (A) particles in suspension to which are bound antibodies of the IgG class, which have affinity for protein A of Staphylococcus, (B) particles in suspension to which are bound fibrinogen, and (C) particles in suspension to which are bound at least one monoclonal antibody which binds to a capsular polysaccharide of type 5 of Staphylococcus aureus.
A method for the detection of Staphylococcus aureus including oxacillin resistant Staphylococcus aureus that escapes detection by agglutination in the presence of fibrinogen and antibodies directed against protein A of Staphylococcus in a sample by agglutination, comprising the steps of:
- (a) mixing the sample with a reagent for the detection by agglutination of Staphylococcus aureus comprising particles in suspension to which are bound fibrinogen, particles in suspension to which are bound antibodies, which have affinity for protein A of Staphylococcus, and particles in suspension to which are bound at least one antibody, which binds to a capsular polysaccharide of type 5 of Staphylococcus aureus, wherein said reagent can agglutinate oxacillin resistant Staphylococcus aureus that escapes detection by agglutination in the presence of only fibrinogen and antibodies directed against protein A of Staphylococcus, and
- (b) detecting Staphylococcus aureus including oxacillin resistant Staphylococcus aureus that escapes detection by agglutination in the presence of fibrinogen and antibodies directed against protein A of Staphylococcus by detecting agglutination in said mixture.
Dependencies are inferred from the claim text.