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AssigneeAmersham Biosciences AB
InventorsTravis Johnson, John McGowen, Allyson Beuhler, Charles Kimball Brush, Robert Emil Lajos
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The present invention provides solid supports (e.g., glass) and polymer hydrogels (particularly polymer hydrogel arrays present on a solid support) comprising one or more reactive sites for the attachment of biomolecules, as well as biomolecules comprising one or more reactive sites for attachment to solid supports and polymer hydrogels. The invention further provides novel compositions and methods for the preparation of biomolecules, solid supports, and polymer hydrogels comprising reactive sites. The invention also provides for preparation of crosslinked solid supports, polymer hydrogels, and hydrogel arrays, wherein one or more biomolecules is attached by means of the reactive sites in a photocycloaddition reaction. Advantageously, according to the invention, crosslinking of the hydrogel and attachment of biomolecules can be done in a single step.
An article comprising one or more biomolecule(s) covalently attached to a polymer-coated solid support, wherein the attachment is by 2+2 cycloaddition between a reactive site present on said polymer and a reactive site present on said biomolecule(s).
An article comprising one or more biomolecule(s) covalently attached to a solid support, wherein the attachment is by 2+2 cycloaddition between a reactive site present on the solid support and a reactive site present on the biomolecule(s).
In a method for attaching one or more biomolecule(s) to a polymer hydrogel or hydrogel array, the improvement comprising attaching said biomolecule(s) to said polymer hydrogel or hydrogel array using a 2+2 photocycloaddition reaction.
In a method for attaching one or more biomolecule(s) to a solid support by covalent attachment, the improvement comprising attaching said biomolecule(s) to said solid support using a 2+2 photocycloaddition reaction.
In a method of manufacturing polymer hydrogel arrays having one or more biomolecules covalently attached thereto, the improvement comprising simultaneously crosslinking said polymer hydrogel and attaching one or more biomolecule(s) to said hydrogel using a 2+2 photocycloaddition reaction.
Dependencies are inferred from the claim text.