Screening of phage displayed peptides without clearing of the cell culture
AssigneeZyomyx, Inc.
InventorsSteffen Nock, Paul D. Kassner
Life of this patent
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Abstract
This invention provides methods for screening populations of phage-displayed polypeptides that are particularly well-suited for high-throughput screening. The methods do not require the clearing of cells from a culture used to obtain the population of phage or other replicable genetic packages. Accordingly, the invention provides methods for forming complexes between a replicable genetic package displaying a polypeptide fusion and a target molecule in an uncleared cell culture containing replicable genetic package. Compositions made up of an uncleared cell culture containing replicable genetic packages displaying a polypeptide fusion and a target molecule are provided in the invention as well.
Claims19
A method for screening a population of replicable genetic packages to obtain replicable genetic packages that display on their surface a fusion protein that specifically binds to a target molecule, the method comprising:
- contacting a target molecule with an uncleared cell culture, wherein said uncleared cell culture comprises:
- (a) replicable genetic packages, each of which displays on its surface a fusion protein that comprises a surface-displayed replicable genetic package polypeptide and an exogenous polypeptide;
- and
- (b) cells in which the replicable genetic packages were amplified prior to contact with the target molecule;
- wherein said replicable genetic packages that specifically bind to said target molecule via said exogenous polypeptide form complexes that comprise the target molecule and the replicable genetic packages.
Dependencies are inferred from the claim text.