Methods for obtaining nucleotide sequences coding for polypeptides specifically active for larvae of S. littoralis
AssigneesInstitut Pasteur, Institut National de la Recherche Agronomique
InventorsVincent Sanchis, Didier Lereclus, Ghislaine Menou, Marguerite-Marie Lecadet, Daniel Martouret, Raymond Dedonder
Life of this patent
Estimating the term…
Abstract
This invention relates to a method for the cloning a polypeptide having larvicidal activity. In particular, the invention relates to vectors, bacterial strains and methods for the cloning and expression of the N-terminal region of a polypeptide toxic against the larvae of Lepidoptera of the Noctuidae family, preferably against S.littoralis.
Claims1
A method for obtaining a nucleotide sequence that codes for the active part of a polypeptide toxic for larvae of S. littoralis, wherein the method comprises:
- (A) hybridizing at least one nucleotide probe to DNA from a strain of B. thuringiensis active against S. littoralis, wherein the nucleotide probe from the 5′ end of a restriction fragment of a gene for δ endotoxin of B. thuringiensis strain aizawa 7-29, and wherein the nucleotide probe comprises the HincII-PstI fragment of the δ endotoxin of B. thuringiensis strain aizawa 7-29 and further comprises the HindIII-HincII restriction fragment of B. thuringiensis strain entomocidus 6-01;
- (B) isolating the DNA from the strain of B. thuringiensis active against S. littoralis that hybridized to the probe;
- (C) cloning the isolated DNA into a vector;
- and
- (D) purifying the vector to thereby obtain the nucleotide sequence that codes for the active part of a polypeptide toxic for larvae of S. littoralis.
Dependencies are inferred from the claim text.