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US 6,686,148 B1Granted
Methods for targeting RNA molecules
AssigneeNuvelo, Inc.
InventorsLing X. Shen, Gregory L. Verdine, James P. Basilion, Vincent P. Stanton, Jr.
Life of this patent
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Abstract
Methods are described for identifying an RNA target for an allele-specific RNA inhibitor by identifying a sequence variance in an RNA encoded by a gene of interest, and determining whether the secondary structure of the RNA comprising the variance differs from that of an otherwise identical RNA not comprising the variance.
Claims9
A method for identifying an RNA target for an allele specific RNA inhibitor, the method comprising:
- a) identifying in a population of subjects at least one sequence variance in an RNA encoded by a gene of interest;
- b) determining whether the secondary structure of the RNA comprising the at least one sequence variance differs from the secondary structure of an otherwise identical RNA not comprising the at least one sequence variance;
- and
- c) determining that the RNA comprising the at least one sequence variance is a target for an allele specific RNA inhibitor if the secondary structure of the RNA comprising the at least one sequence variance differs from the secondary structure of an otherwise identical RNA not comprising the at least one sequence variance.
1 more element
A method for identifying an RNA target for an allele specific agent, the method comprising:
- a) identifying in a population of subjects at least one sequence variance in an RNA encoded by a gene of interest;
- b) determining whether the secondary structure of the RNA comprising the at least one sequence variance differs from the secondary structure of an otherwise identical RNA not comprising the at least one sequence variance;
- c) determining that the RNA comprising the at least one sequence variance is a candidate target for an allele specific agent if the secondary structure of the RNA comprising the at least one sequence variance differs from the secondary structure of an otherwise identical RNA not comprising the at least one sequence variance;
- d) contacting RNA comprising the at least one sequence variance and an otherwise identical RNA not comprising the at least one sequence variance with an allele specific agent;
- e) determining that the RNA comprising the at least one sequence variance is a target for an allele specific agent if the candidate allele specific agent selectively binds to the RNA comprising the at least one sequence variance differs relative to the otherwise identical RNA not comprising the at least one sequence variance.
2 more elements
Dependencies are inferred from the claim text.