Fluorescence detection instrument with reflective transfer legs for color decimation
AssigneeBecton, Dickinson and Company
InventorsClifford A. Oostman, Jr., Barry J. Blasenheim
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AssigneeBecton, Dickinson and Company
InventorsClifford A. Oostman, Jr., Barry J. Blasenheim
Estimating the term…
An optical instrument using a plurality of lasers of different colors with parallel, closely spaced beams to stimulate scattering and fluorescence from fluorescent biological particulate matter, including cells and large molecules. A large numerical aperture objective lens collects fluorescent light while maintaining spatial separation of light stimulated by the different sources. The collected light is imaged into a plurality of fibers, one fiber associated with each optical source, which conducts light to a plurality of arrays of detectors, with each array associated with light from one of the fibers and one of the lasers. A detector array has up to ten detectors arranged to separate and measure colors within relatively narrow bands by decimation of light arriving in a fiber. A large number of detectors is mounted in a compact polygonal arrangement by using reflective transfer legs from multiple beam splitters where the transfer legs arise from a polygonal arrangement of beam splitters in a circumference within the circumferential arrangement of detectors.
An optical instrument for fluorescence analysis of many colors from a target having fluorescent material comprising,
An optical instrument for fluorescence analysis of many colors from a target substance comprising,
An optical instrument for fluorescence analysis comprising,
An optical instrument of the type having one or more input beams impinging upon fluorescent material to be analyzed with fluorescent light collected by a light collector and formed into at least one output beam for analysis, the output beam having a projected optical axis and a plurality of spaced apart dichroic mirrors disposed along the projected optical axis in a manner separating light at each mirror into a reflected beam and a transmitted beam, wherein one of the reflected and transmitted beams is a transfer leg carrying the beam further to the next dichroic mirror and another leg carrying light to a detector wherein the improvement comprises,
An optical instrument for fluorescence analysis of many colors comprising,
Dependencies are inferred from the claim text.