Amplification and detection of organisms of the Chlamydiaceae family

An oligonucleotide selected from the group consisting of CGLB1.0 (SEQ ID NO:

• 5), Rnp LB (SEQ ID NO:
• 6), CGRB1.0 (SEQ ID NO:
• 7) and Rnp RB (SEQ ID NO:
• 8).

An oligonucleotide selected from the group consisting of CGA1.0 (SEQ ID NO:

• 9), a nucleic acid complementary to CGA1.0 (SEQ ID NO:
• 9), Rnp AD (SEQ ID NO:
• 10) and a nucleic acid complementary to Rnp AD (SEQ ID NO:
• 10).

A pair of amplification primers comprising:

• a) a first primer consisting of a target binding sequence selected from the group consisting of the target binding sequences of CGLP1.0 (SEQ ID NO:
• 1) and Rnp LP (SEQ ID NO:
• 2), and, optionally, a sequence required for an amplification reaction, and;
• b) a second primer consisting of a target binding sequence selected from the group consisting of the target binding sequences of CGRP1.0 (SEQ ID NO:
• 3) and Rnp RP (SEQ ID NO:
• 4), and, optionally, a sequence required for an amplification reaction.

A kit comprising:

• a) one or more primers selected from the group consisting of CGLP1.0 (SEQ ID NO:
• 1) and Rnp LP (SEQ ID NO:
• 2),
• b) one or more primers selected from the group consisting of CGRP1.0 (SEQ ID NO:
• 3) and Rnp RP (SEQ ID NO:
• 4),
• c) one or more bumpers selected from the group consisting of CGLB1.0 (SEQ ID NO:
• 5), Rnp LB (SEQ ID NO:
• 6), CGRB1.0 (SEQ ID NO:
• 7) and Rnp RB (SEQ ID NO:
• 8), and
• d) one or more signal primers selected from the group consisting of CGA1.0 (SEQ ID NO:
• 9), a nucleic acid complementary to CGA1.0 (SEQ ID NO:
• 9), Rnp AD (SEQ ID NO:
• 10) and a nucleic acid complementary to Rnp AD (SEQ ID NO:
• 10).

A method for detecting the presence or absence of organisms of the Chlamydiaceae family in a sample, said method comprising:

• a) treating said sample using a pair of nucleic acid primers in a nucleic acid amplification reaction wherein a first primer is selected from the group consisting of CGLP1.0 (SEQ ID NO:
• 1) and Rnp LP (SEQ ID NO:
• 2), and said second primer is selected from the group consisting of CGRP1.0 (SEQ ID NO:
• 3) and Rnp RP (SEQ ID NO:
• 4), and
• b) detecting any amplified nucleic acid product, wherein detection of amplified product indicates presence of organisms of the Chlamydiaceae family.

A method for amplifying a target nucleic acid sequence from an organism of the Chlamydiaceae family comprising:

• a) hybridizing to the nucleic acid
• i) a first amplification primer selected from the group consisting of a target binding sequence of CGLP1.0 (SEQ ID NO:
• 1) and a target binding sequence of Rnp LP (SEQ ID NO:
• 2), and, optionally, a sequence required for an amplification reaction, and
• ii) a second amplification primer selected from the group consisting of a target binding sequence of CGRP1.0 (SEQ ID NO:
• 3) and a target binding sequence of Rnp RP (SEQ ID NO:
• 4), and, optionally, a sequence required for the amplification reaction, and;
• b) extending the hybridized first and second amplification primers on the target nucleic acid sequence whereby the target nucleic acid sequence is amplified.