System and method for improving the accuracy of DNA sequencing and error probability estimation through application of a mathematical model to the analysis of electropherograms

A machine readable medium having stored thereon instructions which when executed by a processor cause a machine to perform operations comprising:

• processing a plurality of information obtained from an electropherogram output by a base calling system;
• creating a plurality of refined base calls using a plurality of original base calls and a plurality of intrinsic peak characteristics;
• assigning a quality value to each of the plurality of refined base calls using the plurality of intrinsic peak characteristics;
• and
• training by generating a training file and look-up table, wherein generating the training file comprises:
• extracting and processing a group of electropherograms from at least one sample file;
• determining called base sequences for each fragment in each electropherogram;
• aligning the called base sequences with a corresponding consensus sequence to identify correct and erroneous base calls;
• computing trace parameters for each base call of the called base sequences;
• and
• writing the base calls as base call data and the trace parameters to the training file.