Hepatitus A virus vaccines
AssigneeThe United States of America as represented by the Department of Health and Human Services
InventorsAnn W. Funkhouser, Suzanne U. Emerson, Robert H. Purcell, Eric D'Hondt
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Abstract
A live hepatitis A virus adapted to growth in MRC-5 cells, which HAV is preferably characterized by suitable attenuation for effective vaccine administration to humans and animals without inactivation, methods for adapting HAV to growth in MRC-5, vaccine compositions and method of vaccinating humans against HAV infection.
Claims10
An isolated DNA molecule encoding a hepatitis A virus adapted to growth in MRC-5 cells, said molecule having a nucleotide sequence which corresponds to the cDNA sequence of HAV HM-175 Pass 35 (SEQ ID NO:
- 3) except for nucleotide positions 591 and 687 which have guanines as bases, nucleotide position 646 which has an adenine as a base and nucleotide position 669 which has a thymine as a base, and optionally at least one nucleotide selected from the group consisting of:
- (a) T at position 2750;
- (b) A at position 3027;
- (c) A at position 3196;
- (d) G at position 3934;
- (e) I at position 4418;
- (f) G at position 4563;
- (g) T at position 4643;
- (h) G at position 5145;
- (i) T at position 5745;
- (j) C at position 6908;
- (k) T at position 7032;
- (l) T at position 7255;
- wherein the nucleotide numbers shown above are those assigned to positions of the wild-type HM-175 sequence.
Dependencies are inferred from the claim text.