Humanized antibody against human tissue factor (TF) and process of production of the humanized antibody
AssigneeChugai Seiyaku Kabushiki Kaisha
InventorsKoh Sato, Hideki Adachi, Naohiro Yabuta
Life of this patent
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Abstract
The present invention relates to a human/mouse chimeric antibody that includes the variable region of a mouse monoclonal antibody against human tissue factor (TF) and the constant region of a human antibody. The humanized antibody contains light and heavy chain complementarity determining regions of a mouse monoclonal antibody against human TF which have been grafted onto a human antibody. The present invention also provides a therapeutic agent for disseminated intravascular coagulation (DIC) syndrome, which includes a humanized antibody against human TF as an active ingredient.
Claims20
A humanized H chain V region consisting of an H chain V region complementarity-determining region (CDRs) of a mouse monoclonal antibody against human tissue factor (TF) and an H chain V region framework region (FRs) of a human antibody, wherein said CDRs consist of the following amino acid sequences:
- H-CDR1:
- Asp Tyr Tyr Met His (SEQ ID NO:
- 133),
- H-CDR2:
- Gly Asn Asp Pro Ala Asn Gly His Ser Met Tyr Asp Pro Lys Phe Gln Gly (SEQ ID NO:
- 134), and
- H-CDR3:
- Asp Ser Gly Tyr Ala Met Asp Tyr (SEQ ID 35 NO:
- 135).
A humanized L chain V region consisting of an L chain V region CDRs of a mouse monoclonal antibody against human TF and a human L chain V region FRs, wherein said CDRs consist of the following amino acid sequences:
- L-CDR1:
- Lys Ala Ser Gln Asp Ile Lys Ser Phe Leu Ser (SEQ ID NO:
- 136),
- L-CDR2:
- Tyr Ala Thr Ser Leu Ala Asp (SEQ ID NO:
- 137), and
- L-CDR3:
- Leu Gln His Gly Glu Ser Pro Tyr Thr (SEQ ID NO:
- 138).
Dependencies are inferred from the claim text.